Human GPR22 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB4500
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Intracellular Staining by Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 455108
Product Specifications
Immunogen
HEK293 human embryonic kidney cell line transfected with human GPR22
Met1-Asp433
Accession # Q99680
Met1-Asp433
Accession # Q99680
Specificity
Detects human GPR22. Stains human GPR22 transfectants but not irrelevant transfectants.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human GPR22 Antibody
Detection of GPR22 in A172 Human Cell Line by Flow Cytometry.
A172 human glioblastoma cell line was stained with Mouse Anti-Human GPR22 Monoclonal Antibody (Catalog # MAB4500, filled histogram) or isotype control antibody (Catalog # MAB0031, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.Applications for Human GPR22 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
Sample: A172 human glioblastoma cell line fixed with paraformaldehyde and permeabilized with saponin
Sample: A172 human glioblastoma cell line fixed with paraformaldehyde and permeabilized with saponin
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GPR22
GPR22 is a 7TM G protein-coupled receptor that is expressed in the brain. It maps to chromosome 7, region q22-q31.1. Human GPR22 shares 97% aa sequence identity with mouse GPR22.
Long Name
G Protein-coupled Receptor 22
Alternate Names
G protein-coupled receptor 22, MGC129847, probable G-protein coupled receptor 22
Gene Symbol
GPR22
UniProt
Additional GPR22 Products
Product Documents for Human GPR22 Antibody
Product Specific Notices for Human GPR22 Antibody
For research use only
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