A172 human glioblastoma cell line was stained with Mouse Anti-Human GPR22 Monoclonal Antibody (Catalog # MAB4500, filled histogram) or isotype control antibody (Catalog # MAB0031, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Applications for Human GPR22 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells
Sample: A172 human glioblastoma cell line fixed with paraformaldehyde and permeabilized with saponin

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GPR22

GPR22 is a 7TM G protein-coupled receptor that is expressed in the brain. It maps to chromosome 7, region q22-q31.1. Human GPR22 shares 97% aa sequence identity with mouse GPR22.

Long Name

G Protein-coupled Receptor 22

Alternate Names

G protein-coupled receptor 22, MGC129847, probable G-protein coupled receptor 22

Entrez Gene IDs

2845 (Human); 73010 (Mouse); 298944 (Rat)

Gene Symbol

GPR22

UniProt

Q99680

Additional GPR22 Products

Product Documents for Human GPR22 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human GPR22 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human GPR22 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB4500

Key Product Details

Species Reactivity

Validated:

Applications

Label

Antibody Source

View all GPR22 Antibodies »

Product Summary for Human GPR22 Antibody

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human GPR22 Antibody

Detection of GPR22 in A172 Human Cell Line by Flow Cytometry.