Human GPR37 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB44502
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 1040226
Product Specifications
Immunogen
Human GPR37 synthetic peptide
Specificity
Detects human GPR37 in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human GPR37 Antibody
GPR37 in Human brain.
GPR37 was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using Mouse Anti-Human GPR37 Monoclonal Antibody (Catalog # MAB44502) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to Purkinje neurons. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human GPR37 Antibody
Application
Recommended Usage
Immunohistochemistry
5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (cerebellum)
Sample: Immersion fixed paraffin-embedded sections of human brain (cerebellum)
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GPR37
GPR37 (G-protein coupled receptor 37), also called ETBR-LP-1 (endothelin B receptor-like protein 1) or PAELR (Parkin-associated endothelin receptor-like receptor) is a 613 aa, ~55 kDa orphan 7-transmembrane receptor. It is mainly expressed in neuronal cells, particularly in cerebellar Purkinje cells and the hippocampus. It is a substrate of the E3 ubiquitin ligase, parkin, which is upregulated during endoplasmic reticulum stress. In a juvenile form of Parkinson’s disease, GPR37 accumulates, contributing to stress-induced neuronal cell death. The extracellular portions of human and mouse GPR37 share 68% aa identity.
Long Name
G Protein-coupled Receptor 37
Alternate Names
ETBR-LP-1, PAELR
Gene Symbol
GPR37
Additional GPR37 Products
Product Documents for Human GPR37 Antibody
Product Specific Notices for Human GPR37 Antibody
For research use only
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