A172 human glioblastoma cell line was stained with Mouse Anti-Human GPR37L1 Monoclonal Antibody (Catalog # MAB4449, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Applications for Human GPR37L1 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells
Sample: A172 human glioblastoma cell line fixed with paraformaldehyde and permeabilized with saponin

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GPR37L1

GPR37L1 (G-protein coupled receptor 37-like 1), also called ETBR-LP-2 (endothelin B receptor-like protein 2), is a 481 aa, orphan 7-transmembrane receptor with calculated molecular weight of 53 kDa. GPR37L1 is expressed in cerebellar Bergmann glia, cerebral cortex and internal capsule fibers in the central nervous system. It shows 39% aa identity with GPR37, a neuronal protein that accumulates in juvenile Parkinson’s disease neurons under endoplasmic reticulum stress. GPR37L1 does not bind known endothelin B family members. The extracellular portions of human and mouse GPR37L1 share 85% aa identity.

Long Name

G Protein-coupled Receptor 37-like 1

Alternate Names

ETBR-LP-2

Entrez Gene IDs

9283 (Human); 171469 (Mouse); 252939 (Rat)

Gene Symbol

GPR37L1

UniProt

O60883

Additional GPR37L1 Products

Product Documents for Human GPR37L1 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human GPR37L1 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human GPR37L1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB4449

Key Product Details

Species Reactivity

Validated:

Applications

Label

Antibody Source

View all GPR37L1 Antibodies »

Product Summary for Human GPR37L1 Antibody

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human GPR37L1 Antibody

Detection of GPR37L1 in A172 Human Cell Line by Flow Cytometry.