HEK293 human embryonic kidney cell line transfected with either (A) human GPR52 or (B) irrelevant protein, and eGFP was stained with Mouse Anti-Human GPR52 Monoclonal Antibody (Catalog # MAB10357) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on isotype control antibody (Catalog # MAB0041, data not shown). View our protocol for Staining Membrane-associated Proteins.

Applications for Human GPR52 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 Human Cell Line Transfected with Human GPR52 and eGFP

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GPR52

GPR52 is a class-A orphan G-protein-coupled receptor (GPCR) that is highly expressed in the brain and represents a promising therapeutic target for the treatment of several neuropsychiatric disorders, including Huntington's disease and schizophrenia. GPR52 is a 41KDa seven-transmembrane domain protein (7TM). Mouse and human sequences are 96% identical over the full protein.

Long Name

G-Protein-Coupled Receptor 52

Alternate Names

G protein-coupled receptor 52, MGC111751, probable G-protein coupled receptor 52

Entrez Gene IDs

9293 (Human); 620246 (Mouse); 680192 (Rat)

Gene Symbol

GPR52

UniProt

Q9Y2T5

Additional GPR52 Products

Product Documents for Human GPR52 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human GPR52 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human GPR52 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB10357

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all GPR52 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human GPR52 Antibody

Detection of GPR52 in HEK293 Human Cell Line Transfected with Human GPR52 and eGFP by Flow Cytometry.