Human GPR85 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB10761
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 1042202
Product Specifications
Immunogen
Human GPR85 extracellular domain synthetic peptide
Specificity
Detects human GPR85 in cell based ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human GPR85 Antibody
GPR85 in U-87 MG Human Cell Line.
GPR85 was detected in immersion fixed U-87 MG human glioblastoma/astrocytoma cell line (positive staining) and A549 human lung carcinoma cell line (negative staining) using Mouse Anti-Human GPR85 Monoclonal Antibody (Catalog # MAB10761) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Human GPR85 Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed U‑87 MG human glioblastoma/astrocytoma cell line
Sample: Immersion fixed U‑87 MG human glioblastoma/astrocytoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GPR85
Long Name
G Protein-coupled Receptor 85
Alternate Names
SREB2
Gene Symbol
GPR85
Additional GPR85 Products
Product Documents for Human GPR85 Antibody
Product Specific Notices for Human GPR85 Antibody
For research use only
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