PC-3 human prostate cancer cell line was stained with Human GPRC5C Monoclonal Antibody (Catalog # MAB6594, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0102B).

Applications for Human GPRC5C Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: PC‑3 human prostate cancer cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GPRC5C

G-protein coupled receptor family C group 5 member C (GPRC5C), also known as Retinoic acid induced gene 3 (RAIG-3), is an approximately 50 kDa protein with seven transmembrane segments and one site of potential N-linked glycosylation. It is predominantly expressed in the stomach, kidney, liver, pancreas, and prostate. Human GPRC5C shares 88% and 90% amino acid sequence identity with mouse and rat GPRC5C, respectively.

Long Name

G Protein-coupled Receptor, Family C, Group 5, Member C

Alternate Names

RAIG3

Entrez Gene IDs

55890 (Human)

Gene Symbol

GPRC5C

UniProt

Q9NQ84

Additional GPRC5C Products

Product Documents for Human GPRC5C Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human GPRC5C Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human GPRC5C Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB6594

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all GPRC5C Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human GPRC5C Antibody

Detection of GPRC5C in PC-3 Human Cell Line by Flow Cytometry.