Human Granzyme B Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF2906
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Intracellular Staining by Flow Cytometry, Western Blot
Cited:
Capture Assay, Flow Cytometry
Label
Biotin
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Granzyme B
Gly19-Tyr247
Accession # P10144
Gly19-Tyr247
Accession # P10144
Specificity
Detects human Granzyme B in Western blots. In Western blots, approximately 25% cross-reactivity with recombinant mouse (rm) Granzyme B and rmGranzyme D is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Human Granzyme B Biotinylated Antibody
Application
Recommended Usage
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
Sample: NK-92 human natural killer lymphoma cell line fixed with paraformaldehyde and permeabilized with saponin
Sample: NK-92 human natural killer lymphoma cell line fixed with paraformaldehyde and permeabilized with saponin
Western Blot
0.1 µg/mL
Sample: Recombinant Human Granzyme B (Catalog # 2906-SE)
Sample: Recombinant Human Granzyme B (Catalog # 2906-SE)
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Granzyme B
Granzyme B is a member of the granzyme family of the serine proteases found specifically in the cytotoxic granules of cytotoxic T lymphocytes (CTL) and natural killer (NK) cells (1, 2). Granzyme B plays an essential role in granule-mediated apoptosis and may have additional roles in rheumatoid arthritis and in bacterial and viral infections (3). It activates various caspases and cleaves proteins such as aggrecan (3). Human Granzyme B is synthesized as a precursor (247 residues) with a signal peptide (residues 1‑18), a pro peptide (residues 19‑20), and a mature chain (residues 21‑247) (4‑6). The rhGranzyme B consisting of residues 19‑247 was expressed and purified. After being activated by active cathepsin C, rhGranzyme B cleaves a thioester substrate described previously (3).
References
- Kam, C.-M. et al. (2000) Biochim. Biophys. Acta 1477:307.
- Smyth, M.J. et al. (1996) J. Leukoc. Biol. 60:555.
- Froelich, C.J. (2004) in Handbook of Proteolytic Enzymes, Barrett, A.J. et al., eds., pp. 1549.
- Schmid, J. and C. Weissman (1987) J. Immunol. 139:250.
- Caputo, A. et al. (1988) J. Biol. Chem. 263:6363.
- Trapani, J.A. et al. (1988) Proc. Natl. Acad. Sci. USA 85:6924.
Alternate Names
CGL-1, CGL1, CSPB, CTLA-1, CTLA1, CTSGL1, Fragmentin-2, Granzyme-2, GRB, GrzB, GZMB, HLP, SECT
Gene Symbol
GZMB
UniProt
Additional Granzyme B Products
Product Documents for Human Granzyme B Biotinylated Antibody
Product Specific Notices for Human Granzyme B Biotinylated Antibody
For research use only
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