Human GRK3 Antibody

Detection of Human GRK3 by Western Blot.

Western blot shows lysates of Jurkat human acute T cell leukemia cell line and CEM human T-lymphoblastoid cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human GRK3 Monoclonal Antibody (Catalog # MAB47851) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for GRK3 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

GRK3 in CEM-NKr Human Cell Line.

GRK3 was detected in immersion fixed CEM-NKr human Natural Killer cell resistant T-lymphoblastoid cell line using Mouse Anti-Human GRK3 Monoclonal Antibody (Catalog # MAB47851) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

GRK3 in Human Prostate Cancer Tissue.

GRK3 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Mouse Anti-Human GRK3 Monoclonal Antibody (Catalog # MAB47851) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.