HEK293 transfected with Human GUCY2C and eGFP were stained with eGFP and either (A) Rabbit Anti-Human Guanylyl Cyclase C/GUCY2C PE-conjugated Monoclonal Antibody (Catalog # FAB2157P) or (B) Rabbit IgG PE-conjugated Antibody (Catalog # IC1051P). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Guanylyl Cyclase C/GUCY2C PE-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: HEK293 transfected with Human GUCY2C and eGFP

Reviewed Applications

Read 1 review rated 5 using FAB2157P in the following applications:

Flow Cytometry (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Formulation

Supplied 0.005 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Guanylyl Cyclase C/GUCY2C

GUCY2C (Guanylyl Cyclase C), also known as heat-stable enterotoxin receptor, is a type I transmembrane protein of the guanylate cyclase (gc) family that signal by producing cGMP (1, 2). GUCY2C contains a 23 amino acid (aa) signal sequence, a 407 aa extracellular region (ECD), a 24 aa transmembrane (TM) segment and a 619 aa cytoplasmic domain (3). The ECD of human GUCY2C shares 71% and 72% aa identity with mouse and rat GUCY2C, respectively (4). GUCY2C was first identified as the intestinal epithelial receptor regulating fluid and electrolyte transport in the secretory diarrhea induced by bacterial enterotoxins (5). Endogenous ligands of GUCY2C include guanylin and uroguanylin (6). GUCY2C in epithelial cells plays an important role in cell dynamics and homeostatic balance of proliferation, metabolism, and differentiation that organizes the guanylyl cyclase C hormone axis (2, 6). GUCY2C is also expressed in the brain and is implicated in attention deficiency and hyperactive behavior (2, 7). CAR-T cell therapy utilizing GUCY2C to treat metastatic colorectal cancer is currently being explored (8).

References

Arshad, N. et al. (2013) J. Biol. Chem. 288:3907.
Gibbons, A. V. et al. (2013) Cancer Res. 73:22.
de Sauvage, F. J. et al. (1991) J. Biol. Chem. 266:17912.
Singh S. et al. (1991) Biochem. Biophys. Res. Comm. 179:1455.
Lucas K. et al. (2000) Pharmacol Rev. 52:375.
Erik, S. et al. (2016) Mol. Pharmacol. 90:199.
Gong, R. et al. (2011) Science. 333:1642.
Magee, MS. et al. (2018) Cancer Immuno Res. 6:509.

Alternate Names

DIAR6, GC-C, GCC, Guanylate Cyclase C, GUC2C, GUCY2C, MUCIL, STA Receptor, StAR

Entrez Gene IDs

2984 (Human); 14917 (Mouse); 25711 (Rat)

Gene Symbol

GUCY2C

UniProt

P25092

Additional Guanylyl Cyclase C/GUCY2C Products

Product Documents for Human Guanylyl Cyclase C/GUCY2C PE-conjugated Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human Guanylyl Cyclase C/GUCY2C PE-conjugated Antibody

For research use only

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Reviews

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Flow Cytometry

Human Guanylyl Cyclase C/GUCY2C PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB2157P

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all Guanylyl Cyclase C/GUCY2C Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Guanylyl Cyclase C/GUCY2C PE-conjugated Antibody

Detection of Guanylyl Cyclase C/GUCY2C in hGUCY2C eGFP/HEK293 tfx cells by Flow Cytometry.