Human HAPLN4 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4085
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Mouse
Applications
Validated:
Immunohistochemistry, Immunoprecipitation, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human HAPLN4
Gln30-Val402
Accession # Q86UW8
Gln30-Val402
Accession # Q86UW8
Specificity
Detects human HAPLN4 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human HAPLN1 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human HAPLN4 Antibody
HAPLN4 in Human Brain.
HAPLN4 was detected in immersion fixed paraffin-embedded sections of human brain (caudate nucleus) using 3 µg/mL Goat Anti-Human HAPLN4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4085) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human HAPLN4 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (caudate nucleus) subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)
Sample: Immersion fixed paraffin-embedded sections of human brain (caudate nucleus) subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human HAPLN4, see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human HAPLN4, see our available Western blot detection antibodies
Western Blot
0.1 µg/mL
Sample: Recombinant Human HAPLN4
Sample: Recombinant Human HAPLN4
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HAPLN4
HAPLN4 (hyaluronan and proteoglycan link protein 4; also Bral2) is a 40-42 kDa secreted member of the Link module superfamily. It is secreted by select neurons, particularly of the cerebellum, where it participates in ECM stability. Here it may link hyaluronic acid with resident proteoglycans. Mature HAPLN4 is 383 amino acids (aa) in length. It contains one lg-like C2-type domain (aa 46-161) and two link domains (aa 163-365). One potential alternate start site exists 36 aa upstream of the standard start site. Human and mouse HAPLN4 share 91% aa sequence identity.
Long Name
Hyaluronan and Proteoglycan Link Protein 4
Alternate Names
BRAL2, Lpr4
Gene Symbol
HAPLN4
UniProt
Additional HAPLN4 Products
Product Documents for Human HAPLN4 Antibody
Product Specific Notices for Human HAPLN4 Antibody
For research use only
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