Skip to main content

Human Hepassocin/FGL1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB112302

R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
Loading...
MAB112302-100
MAB112302-SP

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry, Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1052936

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Hepassocin/FGL1
Met1-Ile312
Accession # BAB70690

Specificity

Detects human Hepassocin/FGL1 in direct ELISA.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Hepassocin/FGL1 Antibody

Detection of Hepassocin/FGL1 in Human Liver.

Hepassocin/FGL1 was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human Hepassocin/FGL1 Monoclonal Antibody (Catalog # MAB112302) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in hepatocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of Hepassocin/FGL1 in HepG2 Human hepatocellular carcinoma cell line (positive) and K562 Human chronic myelogenous leukemia cell line (negative).

Hepassocin/FGL1 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line (positive) and K562 human chronic myelogenous leukemia cell line (negative) using Mouse Anti-Human Hepassocin/FGL1 Monoclonal Antibody (Catalog # MAB112302) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human Hepassocin/FGL1 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HepG2 human hepatocellular carcinoma cell line (positive) and K562 human chronic myelogenous leukemia cell line (negative) 

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Hepassocin/FGL1

Hepassocin, also known as hepatocyte-derived fibrinogen-related protein 1 (HFREP-1) and FGL1, is a liver-specific secreted protein belonging to the fibronogen superfamily, whose members share a fibrinogen domain at their C-termini. Hepassocin may play a role in the development of hepatocellular carcinomas.

Long Name

Fibrinogen Like 1

Alternate Names

FGL1, Fibrinogen-like-protein 1, FREP1

Entrez Gene IDs

2267 (Human); 234199 (Mouse); 246186 (Rat); 102118020 (Cynomolgus Monkey)

Gene Symbol

FGL1

UniProt

Additional Hepassocin/FGL1 Products

Product Documents for Human Hepassocin/FGL1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Hepassocin/FGL1 Antibody

For research use only

Loading...
Loading...
Loading...
Loading...