Human Hepsin Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB4776
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunoprecipitation, Western Blot
Cited:
Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 506220
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Hepsin
Ile163-Leu417
Accession # P05981
Ile163-Leu417
Accession # P05981
Specificity
Detects human Hepsin in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Applications for Human Hepsin Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Hepsin (Catalog # 4776‑SE), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human Hepsin (Catalog # 4776‑SE), see our available Western blot detection antibodies
Western Blot
1 µg/mL
Sample: Recombinant Human Hepsin (Catalog # 4776‑SE) under non-reducing conditions only
Sample: Recombinant Human Hepsin (Catalog # 4776‑SE) under non-reducing conditions only
Reviewed Applications
Read 2 reviews rated 4 using MAB4776 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Hepsin
Hepsin, also known as TMPRSS1, is a Type II membrane protein with an extracellular serine protease domain (1). It is most highly expressed in liver, but is also present in many other tissues, notably lung, kidney, and skeletal muscle (2). A soluble form of Hepsin lacking the transmembrane domain has been identified (3). Hepsin is capable of activating Factor VII, and may initiate blood coagulation at the cell surface (4). Hepsin is overexpressed in various human tumors, including prostate (5), and is considered to be a biomarker for prostate cancer (6). rhHepsin was expressed as a secreted, soluble protein lacking its cytosolic and transmembrane domains. The D161E and R162K mutations were introduced into the prosequence to improve expression of the recombinant human Hepsin.
References
- Leytus, S.P. et al. (1988) Biochemistry 27:1067.
- Tsuji, A. et al. (1991) J. Biol. Chem. 266:16948.
- Li, Y. et al. (2005) Biomed. Biochim. Acta 1681:157.
- Kazama, Y. et al. (1995) J. Biol. Chem. 270:66.
- Dhanasekaran, S.M. et al. (2001) Nature 412:822.
- Wu, Q. and Parry, G. (2007) Front. Biosci. 12:5052.
Alternate Names
HPN, TMPRSS1
Gene Symbol
HPN
UniProt
Additional Hepsin Products
Product Documents for Human Hepsin Antibody
Product Specific Notices for Human Hepsin Antibody
For research use only
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