Human Hepsin Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4776
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Hepsin
Ser46-Leu417 with Asp161Glu and Arg162Lys substitutions
Accession # P05981
Ser46-Leu417 with Asp161Glu and Arg162Lys substitutions
Accession # P05981
Specificity
Detects human Hepsin in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human Hepsin Antibody
Detection of Human Hepsin by Western Blot.
Western blot shows lysates of human prostate tissue, human liver tissue, LNCaP human prostate cancer cell line, and PC-3 human prostate cancer cell line. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human Hepsin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4776) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Hepsin at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 7.Applications for Human Hepsin Antibody
Application
Recommended Usage
Western Blot
2 µg/mL
Sample: Human prostate tissue, human liver tissue, LNCaP human prostate cancer cell line, and PC‑3 human prostate cancer cell line
Sample: Human prostate tissue, human liver tissue, LNCaP human prostate cancer cell line, and PC‑3 human prostate cancer cell line
Reviewed Applications
Read 1 review rated 5 using AF4776 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Hepsin
Hepsin, also known as TMPRSS1, is a Type II membrane protein with an extracellular serine protease domain (1). It is most highly expressed in liver, but is also present in many other tissues, notably lung, kidney, and skeletal muscle (2). A soluble form of Hepsin lacking the transmembrane domain has been identified (3). Hepsin is capable of activating Factor VII, and may initiate blood coagulation at the cell surface (4). Hepsin is overexpressed in various human tumors, including prostate (5), and is considered to be a biomarker for prostate cancer (6).
References
- Leytus, S.P. et al. (1988) Biochemistry 27:1067.
- Tsuji, A. et al. (1991) J. Biol. Chem. 266:16948.
- Li, Y. et al. (2005) Biomed. Biochim. Acta 1681:157.
- Kazama, Y. et al. (1995) J. Biol. Chem. 270:66.
- Dhanasekaran, S.M. et al. (2001) Nature 412:822.
- Wu, Q. and Parry, G. (2007) Front. Biosci. 12:5052.
Alternate Names
HPN, TMPRSS1
Gene Symbol
HPN
UniProt
Additional Hepsin Products
Product Documents for Human Hepsin Antibody
Product Specific Notices for Human Hepsin Antibody
For research use only
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