Human HIN-1/SCGB3A1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB27901
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunocytochemistry, Western Blot
Cited:
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 410219
Product Specifications
Immunogen
E. coli-derived recombinant human HIN‑1/SCGB3A1
Phe21-Gly104
Accession # Q96QR1
Phe21-Gly104
Accession # Q96QR1
Specificity
Detects human HIN‑1/SCGB3A1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross‑reactivity with recombinant mouse HIN-1 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Applications for Human HIN-1/SCGB3A1 Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion-fixed Y-79 human retinoblastoma cell line
Sample: Immersion-fixed Y-79 human retinoblastoma cell line
Western Blot
1 µg/mL
Sample: Recombinant Human HIN-1/SCGB3A1
Sample: Recombinant Human HIN-1/SCGB3A1
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using MAB27901 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HIN-1/SCGB3A1
High in Normal-1 (HIN-1), also known as Secretoglobulin 3A1 (SCGB3A1), PnSP-2, LuLeu-2, and UGRP-2 is an 8 kDa member of the Secretoglobulin family of molecules. It is synthesized as a 104 aa precursor that contains a 21 aa signal sequence and an 83 aa mature region. There are no potential N-linked glycosylation sites. Based on other members of the family, HIN-1 is likely to exist as a disulfide-linked homodimer. The molecule is found in mammary and upper airway epithelium. Mature mouse HIN-1 shares 60% aa sequence identity with mature human HIN-1.
Long Name
High in Normal 1
Alternate Names
HIN1, PnSP-2, SCGB3A1, UGRP2
Gene Symbol
SCGB3A1
UniProt
Additional HIN-1/SCGB3A1 Products
Product Documents for Human HIN-1/SCGB3A1 Antibody
Product Specific Notices for Human HIN-1/SCGB3A1 Antibody
For research use only
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