Human HIPK1 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF5617

R&D Systems, part of Bio-Techne
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AF5617
AF5617-SP
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all HIPK1 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human HIPK1
Gln701-Ser900
Accession # Q86Z02

Specificity

Detects endogenous human HIPK1 in Western blots. Approximately 15% cross reactivity to HIPK2 is observed in Western blot. 

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human HIPK1 Antibody

Detection of Human HIPK1 antibody by Western Blot.

Detection of Human HIPK1 by Western Blot.

Western blot shows lysates of 293T human embryonic kidney cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human HIPK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5617) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for HIPK1 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human HIPK1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: 293T human embryonic kidney cell line and HeLa human cervical epithelial carcinoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HIPK1

HIPK1 (Homeodomain-interacting protein kinase 1) is a 120‑130 kDa member of the HIPK subfamily, CMGC Ser/Thr family of protein kinases. It is ubiquitously expressed, and plays a role in transcriptional regulation and signal transduction. Human HIPK1 is 1210 amino acids (aa) in length. It contains a protein kinase domain (aa 190‑518) that phosphorylates itself as well as Daxx and p53, an interaction region between aa 581‑847, a PEST sequence (aa 889‑973) and a YH domain (aa 1066‑1209) that recruits CtBP. Four different splice variants have been identified. Over aa 701‑900, human and mouse HIPK1 share 97% aa identity.

Long Name

Homeodomain Interacting Protein Kinase 1

Alternate Names

Myak, Nbak2

Entrez Gene IDs

204851 (Human); 15257 (Mouse); 365895 (Rat)

Gene Symbol

HIPK1

UniProt

Q86Z02

Additional HIPK1 Products

Product Documents for Human HIPK1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human HIPK1 Antibody

For research use only

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