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Key Product Details

Species Reactivity

Human

Applications

Intracellular Staining by Flow Cytometry

Label

Alexa Fluor 700 (Excitation = 675-700 nm, Emission = 723 nm)

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2128A

Product Specifications

Immunogen

Human HIST3H3 synthetic peptide

Specificity

Detects human HIST3H3 in Western blots.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Applications for Human HIST3H3 Alexa Fluor® 700-conjugated Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

0.25-1 µg/106 cells
Sample: HeLa Human Cell Line fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: HIST3H3

Histones are alkaline proteins which organize nuclear DNA into nucleosomes. Histone H3 is one of four core histones, which exist as dimers to form an octameric histone core. Epigenetic modification of the exposed histone tails by methylation or acetylation is known to regulate transcription in various ways and is known to affect the etiology of a variety of cancers and developmental defects.  Methylation by methyltranserases mediates transcriptional activation (SET1, Chd1), elongation (MLL, WDR5) or silencing (SET7/9, JMJD2A). Expression of H3K4 methyltransferases have been associated with solid tumors such as prostate, ovary, squamous cell carcinoma, hepatocellular carcinoma and cancers of the blood, including T-cell acute lymphoblastic leukemia, acute myeloid leukemia, acute lymphoblastic leukemia, and others.

Long Name

Histone Cluster 3 H3

Alternate Names

H3.4, H3/G, H3/T, H3FT, H3t, Histone 3, H3

Entrez Gene IDs

8290 (Human)

Gene Symbol

H3-4

Additional HIST3H3 Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human HIST3H3 Alexa Fluor® 700-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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