HEK293 human embryonic kidney cell line transfected with (A) Histamine H3 R or (B) irrelevant protein, and eGFP were stained with Mouse Anti-Human Histamine H3 R Monoclonal Antibody (Catalog # MAB10200) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based Mouse IgG2B Isotype Control Antibody staining (Catalog # MAB0041, data not shown). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Histamine H3 R Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 Human Cell Line Transfected with Human Histamine H3 R and eGFP

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Histamine H3 R

Histamine is a ubiquitous messenger molecule released from mast cells, enterochromaffin-like cells, and neurons. Its various actions are mediated by histamine receptors Histamine H1 R, Histamine H2 R, Histamine H3 R and Histamine H4 R. HRH3 encodes one of the histamine receptors (H3) which belongs to the family 1 of G protein-coupled receptors. Histamine H3 R is an integral membrane protein and can regulate neurotransmitter release. It can also increase voltage-dependent calcium current in smooth muscles and innervates the blood vessels and the heart in cardiovascular system.

Long Name

Histamine H3 Receptor

Alternate Names

GPCR97, HH3R, Histamine H3R, HRH3

Entrez Gene IDs

11255 (Human); 99296 (Mouse)

Gene Symbol

HRH3

UniProt

Q9Y5N1

Additional Histamine H3 R Products

Product Documents for Human Histamine H3 R Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Histamine H3 R Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human Histamine H3 R Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB10200

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all Histamine H3 R Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Histamine H3 R Antibody

Detection of Histamine H3 R in HEK293 Human Cell Line Transfected with Human Histamine H3 R and eGFP by Flow Cytometry.