Human HLA-C Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB10394
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG Clone # DT-9
Product Specifications
Immunogen
Purified tamarin MHC class I molecules (using anti-class I antibody BB7.7)
Accession # P10321
Accession # P10321
Specificity
Detects human HLA-C in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG
Scientific Data Images for Human HLA-C Antibody
Detection of HLA-C in Human PBMC Lymphocytes by Flow Cytometry.
Human PBMC lymphocytes were stained with Mouse Anti-Human HLA-C Monoclonal Antibody (Catalog # MAB10394, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by PE-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.Applications for Human HLA-C Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: Human PBMC Lymphocytes
Sample: Human PBMC Lymphocytes
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HLA-C
HLA-A, B, and C are approximately 45 kDa transmembrane glycoproteins in the major histocompatibility complex 1 (MHC I) family. They contain three alpha domains in their extracellular regions. HLA molecules are expressed on nearly all nucleated cells in association with the 12 kDa beta 2-Microglobulin. This complex binds peptides derived from pathogenic cytosolic or extracellular proteins such as viral or microbial proteins. It presents these peptides on the cell surface for recognition by the T cell receptor on CD8+ cytotoxic T cells. The activated cytotoxic T cell then kills the presenting cell. Mismatched MHC I alleles between a host and a donor lead to transplant rejection.
Long Name
Major Histocompatibility Complex, Class I, C
Alternate Names
D6S204, HLA-JY3, HLAC, HLC-C
Entrez Gene IDs
3107 (Human)
Gene Symbol
HLA-C
UniProt
Additional HLA-C Products
Product Documents for Human HLA-C Antibody
Product Specific Notices for Human HLA-C Antibody
For research use only
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