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Human HNF-3 beta/FoxA2 Alexa Fluor® 488-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # IC2400G

R&D Systems, part of Bio-Techne
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IC2400G

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Immunocytochemistry, Intracellular Staining by Flow Cytometry

Cited:

Flow Cytometry, Immunocytochemistry, Immunohistochemistry

Label

Alexa Fluor 488 (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Polyclonal Goat IgG

Product Specifications

Immunogen

E. coli-derived recombinant human HNF‑3 beta/FoxA2
Met242-Ser457
Accession # Q9Y261

Specificity

Detects human HNF‑3 beta/FoxA2 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human HNF-3 beta/FoxA2 Alexa Fluor® 488-conjugated Antibody

HNF-3 beta/Fox A2 antibody in Mesendoderm Differentiated BG01V Human Stem Cells by Immunocytochemistry (ICC).

HNF-3 beta/Fox A2 in Mesendoderm Differentiated BG01V Human Stem Cells.

BG01V human embryonic stem cells were differentiated into mesendoderm using the StemXVivo Endoderm Kit (Catalog # SC019). HNF-3 beta/FoxA2 was detected in immersion fixed mesendoderm differentiated BG01V human embryonic stem cells using 10 µg/mL Goat Anti-Human HNF-3 beta/FoxA2 Alexa Fluor® 488-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC2400G, green) for 3 hours at room temperature and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of HNF-3 beta/FoxA2 antibody in Human iPS Cells antibody by Flow Cytometry.

Detection of HNF‑3 beta/FoxA2 in Human iPS Cells by Flow Cytometry.

Human iPS cells were stained with Goat Anti-Human HNF-3 beta/FoxA2 Alexa Fluor® 488-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC2400G, filled histogram) or isotype control antibody (Catalog # IC108G, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Detection of HNF‑3 beta/FoxA2 in HepG2 Human Cell Line by Flow Cytometry

HepG2 human liver cell line was stained with Goat Anti-Human HNF-3 beta/FoxA2 Alexa Fluor® 488-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC2400G, filled histogram) or isotype control antibody (IC108G, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human HNF-3 beta/FoxA2 Alexa Fluor® 488-conjugated Antibody

Application
Recommended Usage

Immunocytochemistry

10 µg/mL
Sample: Immersion fixed mesendoderm differentiated BG01V human embryonic stem cells

Intracellular Staining by Flow Cytometry

5 µL/106 cells
Sample: Human HepG2 cell line and iPS cells fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: HNF-3 beta/FoxA2

HNF-3 beta, also known as FoxA2, is a member of the forkhead class of DNA-binding proteins. It is a transcriptional activator for liver-specific transcripts such as albumin and transthyretin. Similar family members play roles in the differentiation of the pancreas and liver.

Long Name

Hepatocyte Nuclear Factor-3, beta

Alternate Names

FoxA2, HNF3 beta

Entrez Gene IDs

3170 (Human)

Gene Symbol

FOXA2

UniProt

Additional HNF-3 beta/FoxA2 Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human HNF-3 beta/FoxA2 Alexa Fluor® 488-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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