Human HNF-4 gamma/NR2A2 MAb (Clone N3224)
R&D Systems, part of Bio-Techne | Catalog # PP-N3224-00
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # N3224
Product Specifications
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human HNF-4 gamma/NR2A2 MAb (Clone N3224)
HNF-4 gamma/NR2A2 in Rat Small Intestine.
HNF-4 gamma/NR2A2 was detected in perfusion fixed paraffin-embedded sections of rat small intestine using HNF-4 gamma/NR2A2 Monoclonal Antibody (Catalog # PP-N3224-00) overnight at 4 °C. Tissue was stained (brown) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human HNF-4 gamma/NR2A2 MAb (Clone N3224)
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Perfusion fixed paraffin-embedded sections of rat small intestine
Sample: Perfusion fixed paraffin-embedded sections of rat small intestine
Western Blot
Reviewed Applications
Read 1 review rated 5 using PP-N3224-00 in the following applications:
Formulation, Preparation, and Storage
Formulation
A liquid formulation in physiologic saline with 0.1% NaN3.
Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Background: HNF-4 gamma/NR2A2
Hepatocyte Nuclear Factor 4 alpha (HNF4 alpha; NR2A1) is a member of the orphan nuclear receptor family. HNF4 alpha is expressed in the liver, kidney, intestine and pancreas. Mutation of HNF4 alpha in humans has been associated with maturity-onset diabetes of the young type 1 (MODY1). HNF4 binds to DNA as an exclusive homodimer. The HNF4 alpha gene is alternatively spliced and may generate up to nine different isoforms, HNF4 alpha 1 through HNF4 alpha 9. The expression of HNF-4 gamma (NR2A2) is observed in the pancreas, kidney, small intestine, and testes with no expression detected in the liver. The level of HNF-4 gamma is less 10% than that of HNF-4 alpha.
Long Name
Hepatocyte Nuclear Factor 4 gamma
Alternate Names
HNF4 gamma, HNF4G, NR2A2
Entrez Gene IDs
3174 (Human)
Gene Symbol
HNF4G
Additional HNF-4 gamma/NR2A2 Products
Product Documents for Human HNF-4 gamma/NR2A2 MAb (Clone N3224)
Product Specific Notices for Human HNF-4 gamma/NR2A2 MAb (Clone N3224)
For research use only
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