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Human Hormone-sensitive Lipase/HSL Antibody

R&D Systems, part of Bio-Techne | Catalog # AF7104

R&D Systems, part of Bio-Techne
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AF7104
AF7104-SP

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

Product Specifications

Immunogen

E. coli-derived recombinant human Hormone-sensitive Lipase/HSL
Met302-Leu425
Accession # Q05469

Specificity

Detects human Hormone-sensitive Lipase/HSL in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human Hormone-sensitive Lipase/HSL Antibody

Detection of Human Hormone-sensitive Lipase/HSL antibody by Western Blot.

Detection of Human Hormone-sensitive Lipase/HSL by Western Blot.

Western blot shows lysates of human heart tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Hormone-sensitive Lipase/HSL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7104) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Hormone-sensitive Lipase/HSL at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Hormone-sensitive Lipase/HSL Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Human heart tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Hormone-sensitive Lipase/HSL

LIPE (also known as hormone sensitive lipase/HSL) is a 88-90 kDa member of the GDXG lipolytic enzyme family of molecules. It is expressed in multiple cell types, including skeletal muscle, adrenal gland and adipocytes, and is regulated by a series of phosphorylations. Catecholamines activate the enzyme via PKA through the phosphorylation of Ser853, Ser855, and Ser951, while insulin depresses its activity via PDE3B. LIPE acts on triglycerides to release free fatty acids, and serves as a retinyl ester hydrolase. Human LIPE is 1076 amino acids (aa) in length, and contains one HSL domain (aa 302-616). This restricted 116 kDa isoform is found in testis (spermatids). There are additional isoform variants. One is 88-90 kDa in size in SDS-Page and possesses an alternative start site to the long form at Met302. This is considered the standard and most common LIPE isoform. A second is approximately 93 kDa in size and contains a 39 aa substitution for aa 1-294. A third isoform is 80 kDa in size and shows a 76 aa deletion in the center of the molecule, reducing its enzymatic activity. It is suggested that LIPE may act as both a monomer and homodimer. Over aa 302-425, human HSL shares 92-93% aa identity with both mouse and rat LIPE.

 

Alternate Names

Hormonesensitive Lipase, HSL, LHS, LIPE

Entrez Gene IDs

3991 (Human); 16890 (Mouse); 25330 (Rat)

Gene Symbol

LIPE

UniProt

Additional Hormone-sensitive Lipase/HSL Products

Product Documents for Human Hormone-sensitive Lipase/HSL Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Hormone-sensitive Lipase/HSL Antibody

For research use only

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