Human HSP47 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB91661
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunocytochemistry, Immunohistochemistry, Western Blot
Cited:
Immunocytochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 950811
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human HSP47
Ala19-Asp412
Accession # P50454
Ala19-Asp412
Accession # P50454
Specificity
Detects human HSP47 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with mouse HSP47 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human HSP47 Antibody
Detection of Human HSP47 by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, A549 human lung carcinoma cell line, and OVCAR-3 human ovarian carcinoma cell line. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human HSP47 Monoclonal Antibody (Catalog # MAB91661) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for HSP47 at approximately 47 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
HSP47 in BG01V Human Embryonic Stem Cells.
HSP47 was detected in immersion fixed BG01V human embryonic stem cells differentiated to hepatocytes using Mouse Anti-Human HSP47 Monoclonal Antibody (Catalog # MAB91661) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
HSP47 in Human Breast Cancer Tissue.
HSP47 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human HSP47 Monoclonal Antibody (Catalog # MAB91661) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human HSP47 Antibody
Application
Recommended Usage
Immunocytochemistry
5-25 µg/mL
Sample: Immersion fixed BG01V human embryonic stem cells differentiated to hepatocytes
Sample: Immersion fixed BG01V human embryonic stem cells differentiated to hepatocytes
Immunohistochemistry
5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue
Western Blot
0.1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, A549 human lung carcinoma cell line, and OVCAR‑3 human ovarian carcinoma cell line
Sample: HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, A549 human lung carcinoma cell line, and OVCAR‑3 human ovarian carcinoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using MAB91661 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HSP47
References
- Christiansen HE, et al, (2010) Am. J. Hum. Genet. 86:3892.
- Tasab M, et al, (2000) EMBO J. 19:22043.
- Kwon YJ, et al, (2009) Oncol Res. 18:1414.
- Zhu J, et al, (2015) Cancer Res. 75:15805.
- Nese N, et al, (2010) Anal Quant Cytol Histol. 32:90
Long Name
Heat Shock Protein 47
Alternate Names
AsTP3, BERF-1, CBP1, CBP2, Colligin, gp46, OI10, PPROM, RA-A47, Serpin H1, SERPINH1
Gene Symbol
SERPINH1
UniProt
Additional HSP47 Products
Product Documents for Human HSP47 Antibody
Product Specific Notices for Human HSP47 Antibody
For research use only
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