Human ICAM-1/CD54 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB720
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
ELISA Capture (Matched Antibody Pair), Immunocytochemistry, Western Blot
Cited:
ELISA Development, ELISA Microarray Development, Luminex Development
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 14C11
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human ICAM-1
Extracellular domain
Extracellular domain
Specificity
Detects recombinant human (rh)ICAM-1 in direct ELISAs and Western blots. In Western blots, shows no cross-reactivity with rrICAM-1, rhICAM-2, rhICAM-3, rhVCAM-1, and rmDCC.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human ICAM-1/CD54 Antibody
ICAM‑1/CD54 in A431 Human Cell Line.
ICAM-1/CD54 was detected in immersion fixed A431 human epithelial carcinoma cell line using Mouse Anti-Human ICAM-1/CD54 Monoclonal Antibody (Catalog # MAB720) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human ICAM-1/CD54 Antibody
Application
Recommended Usage
Immunocytochemistry
5-25 µg/mL
Sample: Immersion fixed A431 human epithelial carcinoma cell line
Sample: Immersion fixed A431 human epithelial carcinoma cell line
Western Blot
1 µg/mL
Sample: Recombinant Human ICAM-1/CD54 (Catalog # ADP4)
under non-reducing conditions only
Sample: Recombinant Human ICAM-1/CD54 (Catalog # ADP4)
under non-reducing conditions only
Human ICAM-1/CD54 Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 3 reviews rated 4.7 using MAB720 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ICAM-1/CD54
Long Name
Intercellular Adhesion Molecule 1
Alternate Names
CD54, ICAM1
Gene Symbol
ICAM1
Additional ICAM-1/CD54 Products
Product Documents for Human ICAM-1/CD54 Antibody
Product Specific Notices for Human ICAM-1/CD54 Antibody
For research use only
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