Human ICAM-1/CD54 Fluorescein-conjugated Antibody

Detection of ICAM‑1/CD54 in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD14 APC-conjugated Monoclonal Antibody (Catalog # FAB3832A) and either (A) Mouse Anti-Human ICAM-1/CD54 Fluorescein-conjugated Monoclonal Antibody (Catalog # BBA20) or (B) Mouse IgG₁Fluorescein Isotype Control (Catalog # IC002F). View our protocol for Staining Membrane-associated Proteins.

Detection of Human ICAM-1/CD54 by Flow Cytometry

EC and PC ICAM-1 expression and effects of CD18 blocking on neutrophil adhesion.ICAM-1 expression on (A) EC or (D) PC monolayers before and after 4 hr IL-1 beta activation was assessed by flow cytometry (IgG labeled control white). ICAM-1 expression on (B,C) EC and (E,F) PC following 4 hr IL-1 beta activation was imaged using confocal microscopy. Scale bars are 20 µm. (G) Neutrophil adhesion inhibition to IL-1 beta activated EC or PC monolayers. Freshly isolated neutrophils were pre-incubated with anti-Mac-1 or anti-LFA-1 antibodies and seeded onto EC or PC monolayers (activated for 4 with IL-1 beta) in Sykes-Moore chambers and allowed to adhere prior to counting. Bars represent average neutrophil adhesion ± SEM. *P<0.05, when compared to the no block control. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0060025), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Human ICAM-1/CD54 by Flow Cytometry

EC and PC ICAM-1 expression and effects of CD18 blocking on neutrophil adhesion.ICAM-1 expression on (A) EC or (D) PC monolayers before and after 4 hr IL-1 beta activation was assessed by flow cytometry (IgG labeled control white). ICAM-1 expression on (B,C) EC and (E,F) PC following 4 hr IL-1 beta activation was imaged using confocal microscopy. Scale bars are 20 µm. (G) Neutrophil adhesion inhibition to IL-1 beta activated EC or PC monolayers. Freshly isolated neutrophils were pre-incubated with anti-Mac-1 or anti-LFA-1 antibodies and seeded onto EC or PC monolayers (activated for 4 with IL-1 beta) in Sykes-Moore chambers and allowed to adhere prior to counting. Bars represent average neutrophil adhesion ± SEM. *P<0.05, when compared to the no block control. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0060025), licensed under a CC-BY license. Not internally tested by R&D Systems.