Human ICAM-3/CD50 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB813
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Mouse
Applications
Validated:
Adhesion Blockade, Western Blot
Cited:
Neutralization
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 76205
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human ICAM‑3/CD50
Gln30-His485
Accession # CAA49473
Gln30-His485
Accession # CAA49473
Specificity
Detects human ICAM-3/CD50 in direct ELISAs and Western blots. Does not cross-react with recombinant human (rh) ICAM‑1 or rhICAM-2.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Applications for Human ICAM-3/CD50 Antibody
Application
Recommended Usage
Adhesion Blockade
The adhesion of HSB2 human peripheral blood acute lymphoblastic leukemia cells (5 x 104 cells/well) to immobilized Recombinant Human ICAM-3/CD50 Fc Chimera (Catalog # 715-IC, 12.5 µg/mL, 100 µL/well) was maximally inhibited (80-100%) by 25 µg/mL of the antibody.
Western Blot
1 µg/mL
Sample: Recombinant Human ICAM-3/CD50 Fc Chimera (Catalog # 715-IC)
Sample: Recombinant Human ICAM-3/CD50 Fc Chimera (Catalog # 715-IC)
Formulation, Preparation, and Storage
Purification
Protein A or G purified from ascites
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ICAM-3/CD50
Intercellular Adhesion Molecule-3 (ICAM-3; also known as CD50), a member of the immunoglobulin superfamily, binds the leukocyte integrins LFA-1 (CD11a/CD18) and alphad/ beta2. ICAM‑3 is expressed on leukocytes and epidermal Langerhans cells. It may play an important role in T cell stimulation by Langerhans cells. ICAM-3 has not been identified in the mouse.
References
- Hayflick, J. et al. (1998) Immunologic Res. 17:313.
Long Name
Intercellular Adhesion Molecule 3
Alternate Names
CD50, ICAM3
Entrez Gene IDs
3385 (Human)
Gene Symbol
ICAM3
UniProt
Additional ICAM-3/CD50 Products
Product Documents for Human ICAM-3/CD50 Antibody
Product Specific Notices for Human ICAM-3/CD50 Antibody
For research use only
Loading...
Loading...
Loading...
Loading...