Human red blood cells were stained with Mouse Anti-Human ICAM-4 APC-conjugated Monoclonal Antibody (Catalog # FAB8397A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human ICAM-4 APC-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: Human red blood cells

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: ICAM-4

ICAM-4 (Intercellular Adhesion Molecule 4), also known as Landsteiner-Wiener Glycoprotein and CD242, is a 42 kDa member of the ICAM family, Ig superfamily of proteins. It is expressed on erythrocytes and erythroblasts, and serves as a receptor for LFA-1, Mac-1, and CD11c/CD18, plus alpha4 beta1 and alpha-V containing integrins. ICAM-4 is suggested to bind to Mac-1 on macrophages, allowing for its phagocytosis in senescence. Mature human ICAM-4 is a 249 amino acid (aa) type I transmembrane glycoprotein. It possesses a 218 aa extracellular region (aa 23-240) that contains two C2-type Ig-like domains (aa 62-124 and 146-217), and a 10 aa C-terminal cytoplasmic tail. ICAM-4 may form 85 kDa homodimers. There are three potential isoform variants. One shows a five aa substitution for aa 233-271, a second contains a 15 aa substitution for aa 14-29, and a third possesses a 141 aa substitution for aa 132-271. Over aa 31-240, human ICAM-4 shares 71% aa identity with mouse ICAM-4.

Long Name

Intercellular Adhesion Molecule 4

Alternate Names

CD242, ICAM4

Entrez Gene IDs

3386 (Human); 78369 (Mouse)

Gene Symbol

ICAM4

UniProt

Q14773 (Human)

Additional ICAM-4 Products

Product Documents for Human ICAM-4 APC-conjugated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human ICAM-4 APC-conjugated Antibody

For research use only

Citations
Reviews

Spectra Viewer
Flow Cytometry

Human ICAM-4 APC-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB8397A

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all ICAM-4 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human ICAM-4 APC-conjugated Antibody

Detection of ICAM-4 in Human Red Blood Cells by Flow Cytometry.