Human IFN-alpha Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB93454

R&D Systems, part of Bio-Techne
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MAB93454-100
MAB93454-SP
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 995514

View all IFN-alpha Antibodies »

Product Specifications

Immunogen

HEK293 human embryonic kidney cell line transfected with human IFN‑ alpha2
Met1-Glu188
Accession # P01563

Specificity

Detects human IFN‑ alpha2a,4a,6,7,8,10,16, and 17 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human IFN-alpha Antibody

IFN-a antibody in Human PBMCs and MCF-7 Human Cell Line by Immunocytochemistry (ICC).

IFN‑ alpha in Human PBMCs and MCF-7 Human Cell Line.

IFN-a was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs; left panel, positive stain) and MCF-7 human breast cancer cell line (right panel, negative stain) using Mouse Anti-Human IFN-a Monoclonal Antibody (Catalog # MAB93454) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human IFN-alpha Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells (PBMCs)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IFN-alpha

Interferon-alpha (IFN-alpha or IFN-alpha), also known as leukocyte interferon, represents a group of related but distinct proteins that share over 95% amino acid sequence homology. They are members of the type I interferon family which share a common cell surface receptor composed of two subunits, a 100 kDa ligand-binding subunit (IFN-alpha R2) and a 125 kDa ligand binding and signal transduction subunit (IFN-alpha R1) that is involved both in ligand binding and signal transduction. Many IFN-alpha subtypes exist, IFN-alpha 1 to IFN alpha-21. The major subtypes identified are IFN-alpha 1, IFN-alpha 2, IFN-alpha 8, IFN-alpha 10, IFN-alpha 14 and IFN-alpha 21. MAB93454 detects human IFN-alpha 2a,4a,6,7,8,10,16, and 17 in direct ELISA.

Long Name

Interferon alpha

Alternate Names

IFNalpha, LeIF D

Entrez Gene IDs

3440 (Human)

Gene Symbol

IFNA2

UniProt

P01563

Additional IFN-alpha Products

Product Documents for Human IFN-alpha Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human IFN-alpha Antibody

For research use only

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