Human IFN-alpha Antibody

Validated:

Cited:

Validated:

Cited:

Unconjugated

Monoclonal Mouse IgG₁ Clone # MMHA-11

Human Leukocyte Interferon

One neutralization unit is the amount of antiserum required to neutralize one unit of human interferon alpha (Hu-IFN- alpha) to a 50% endpoint. Interferon was titrated with the use of the cytopathic effect inhibition assay as described [Rubinstein, S., Familletti, P.C., and Pestka, S. (1981) "Convenient Assay for Interferons," J. Virol . 37, 755-758; Familletti, P.C., Rubinstein, S., and Pestka, S. (1981) "A Convenient and Rapid Cytopathic Effect Inhibition Assay for Interferon," in Methods in Enzymology, Vol. 78 (S. Pestka, ed.), Academic Press, New York, 387-394]. In this antiviral assay for interferon, about 1 unit/ml of interferon is the quantity necessary to produce a cytopathic effect of 50%. The units are determined with respect to the international reference standard for Hu-IFN- alpha provided by the National Institutes of Health [see Pestka, S. (1986) "Interferon Standards and General Abbreviations," in Methods in Enzymology (S. Pestka, ed.), Academic Press, New York 119, 14-23].

Monoclonal

Mouse

IgG₁

<1.0 EU per 1 μg of the antibody by the LAL method.

Protein A or G purified from hybridoma culture supernatant

Phosphate-buffered saline (PBS) containing 0.1% bovine serum albumin (BSA)

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

After receipt, the product may be stored at -20°C for short-term use ( ≤ 6 months). For long-term storage, we recommend storing the product at -70°C or below for retention of full activity. When thawing, the contents of the tube should be apportioned in separate tubes so that freezing and thawing is kept to a minimum. Refreezing should be done on dry ice or in a dry ice/alcohol bath. Further dilution of the product should be in buffers containing protein such as 0.1% bovine serum albumin (BSA).