Human IFN-alpha Antibody
R&D Systems, part of Bio-Techne | Catalog # 21100-2
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
ELISA, Neutralization, Western Blot
Cited:
Surface Plasmon Resonance
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # MMHA-2
Product Specifications
Immunogen
Human Leukocyte Interferon
Specificity
Neutralizes human interferon alpha; binds to human interferon alpha with high affinity (KA ≈ 109 M-1)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Endotoxin Level
<1.0 EU per 1 μg of the antibody by the LAL method.
Applications for Human IFN-alpha Antibody
Application
Recommended Usage
ELISA
Optimal dilutions should be determined by each laboratory for each application.
Western Blot
Optimal dilutions should be determined by each laboratory for each application.
Neutralization
Assay Used to Measure Bioactivity:
One neutralization unit is the amount of antiserum required to neutralize one unit of
human interferon alpha (Hu-IFN-
alpha) to a 50% endpoint. Interferon was titrated with the use of the cytopathic effect
inhibition assay as described [Rubinstein, S., Familletti, P.C., and Pestka, S. (1981) "Convenient Assay for Interferons,"
J.
Virol
. 37, 755-758; Familletti, P.C., Rubinstein, S., and Pestka, S. (1981) "A Convenient and Rapid Cytopathic Effect
Inhibition Assay for Interferon," in
Methods in Enzymology, Vol. 78 (S. Pestka, ed.), Academic Press, New York, 387-394].
In this antiviral assay for interferon, about 1 unit/ml of interferon is the quantity necessary to produce a cytopathic effect of
50%. The units are determined with respect to the international reference standard for Hu-IFN-
alpha provided by the National
Institutes of Health [see Pestka, S. (1986) "Interferon Standards and General Abbreviations," in
Methods in Enzymology
(S. Pestka, ed.), Academic Press, New York 119, 14-23].
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
50 mM Sodium Citrate; 50 mM Sodium Phosphate, pH 7.0
Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
After receipt, the product may be stored at -20°C for short-term use (≤ 6 months). For
long-term storage, we recommend storing the product at -70°C or below for retention of full activity. When thawing, the
contents of the tube should be apportioned in separate tubes so that freezing and thawing is kept to a minimum. Refreezing
should be done on dry ice or in a dry ice/alcohol bath. Further dilution of the product should be in buffers
containing protein such as 0.1% bovine serum albumin (BSA).
Background: IFN-alpha
Long Name
Interferon alpha
Alternate Names
IFNalpha, LeIF D
Entrez Gene IDs
3440 (Human)
Gene Symbol
IFNA2
Additional IFN-alpha Products
Product Documents for Human IFN-alpha Antibody
Product Specific Notices for Human IFN-alpha Antibody
For research use only
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