Human IFN-alpha Antibody
R&D Systems, part of Bio-Techne | Catalog # 21127-1
Key Product Details
Species Reactivity
Human
Applications
Neutralization
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # MMHA-9
Product Specifications
Immunogen
Recombinant human Interferon Alpha D
Specificity
Neutralizes human interferon alpha; binds to human interferon alpha
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Endotoxin Level
<1.0 EU per 1 μg of the antibody by the LAL method.
Applications for Human IFN-alpha Antibody
Application
Recommended Usage
Neutralization
One neutralization unit is the amount of antiserum required to neutralize one unit of
human interferon alpha (Hu-IFN-
alpha) to a 50% endpoint. Interferon was titrated with the use of the cytopathic effect
inhibition assay as described [Rubinstein, S., Familletti, P.C., and Pestka, S. (1981) "Convenient Assay for Interferons,"
J.
Virol
. 37, 755-758; Familletti, P.C., Rubinstein, S., and Pestka, S. (1981) "A Convenient and Rapid Cytopathic Effect
Inhibition Assay for Interferon," in
Methods in Enzymology, Vol. 78 (S. Pestka, ed.), Academic Press, New York, 387-394].
In this antiviral assay for interferon, about 1 unit/ml of interferon is the quantity necessary to produce a cytopathic effect of
50%. The units are determined with respect to the international reference standard for Hu-IFN-
alpha provided by the National
Institutes of Health [see Pestka, S. (1986) "Interferon Standards and General Abbreviations," in
Methods in Enzymology
(S. Pestka, ed.), Academic Press, New York 119, 14-23].
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Phosphate-buffered saline (PBS) containing 0.1% bovine serum albumin (BSA)
Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
After receipt, the product may be stored at -20°C for short-term use (
≤ 6 months). For
long-term storage, we recommend storing the product at -70°C or below for retention of full activity. When thawing, the
contents of the tube should be apportioned in separate tubes so that freezing and thawing is kept to a minimum. Refreezing
should be done on dry ice or in a dry ice/alcohol bath. Further dilution of the product should be in buffers
containing protein such as 0.1% bovine serum albumin (BSA).
Background: IFN-alpha
Long Name
Interferon alpha
Alternate Names
IFNalpha, LeIF D
Entrez Gene IDs
3440 (Human)
Gene Symbol
IFNA2
Additional IFN-alpha Products
Product Documents for Human IFN-alpha Antibody
Product Specific Notices for Human IFN-alpha Antibody
For research use only
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