Human IFN-alpha Antibody
R&D Systems, part of Bio-Techne | Catalog # 31101-1
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Mouse
Applications
Validated:
Neutralization
Cited:
ELISA Development
Label
Unconjugated
Antibody Source
Polyclonal Rabbit Serum
Product Specifications
Immunogen
E. coli-derived recombinant human IFN-alpha
Specificity
Human IFN-alpha
Clonality
Polyclonal
Host
Rabbit
Isotype
Serum
Applications for Human IFN-alpha Antibody
Application
Recommended Usage
Neutralization
One neutralization unit is the amount of antiserum required to neutralize one unit of human interferon alpha (Hu-IFN-alpha) to a 50% endpoint. Interferon was titrated with the use of the cytopathic effect inhibition assay [Rubinstein, S., Familletti, P.C., and Pestka, S. (1981) J. Virol. 37, 755-758] using A549 cells and EMCV was used as the challenge virus [Budd et al. (1985) Canc. Chem. Pharm. 12:39]. In this antiviral assay for interferon, about 1 unit/ml of interferon is the quantity necessary to produce a cytopathic effect of 50%. The units are determined by use of a laboratory standard calibrated with respect to the international reference standard for Hu-IFN-alpha provided by the National Institutes of Health [Gxa01-901-535]. This material is prepared specifically for effective neutralization of Hu-IFN-alpha.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
N/A
Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
After receipt, the product may be stored at -20°C for short-term use (≤ 6 months). For long-term storage, we recommend storing the product at -70°C or below for retention of full activity. When thawing, the contents of the tube should be apportioned in separate tubes so that freezing and thawing is kept to a minimum. Refreezing should be done on dry ice or in a dry ice/alcohol bath. Further dilution of the product should be in buffers containing protein such as 0.1% bovine serum albumin (BSA).
Background: IFN-alpha
Long Name
Interferon alpha
Alternate Names
IFNalpha, LeIF D
Entrez Gene IDs
3440 (Human)
Gene Symbol
IFNA2
Additional IFN-alpha Products
Product Documents for Human IFN-alpha Antibody
Product Specific Notices for Human IFN-alpha Antibody
For research use only
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