Human IFN-alpha Antibody
R&D Systems, part of Bio-Techne | Catalog # 31110-1
Key Product Details
Species Reactivity
Human
Applications
Neutralization
Label
Unconjugated
Antibody Source
Polyclonal Sheep Serum
Product Specifications
Immunogen
Mixture of all recombinant human interferon alpha subtypes (IFNA2, IFNA8, IFNA10, IFNA1, IFNA21, IFNA5, IFNA14, IFNA17, IFNA7, IFNA6, IFNA4, IFNA16)
Specificity
Binds to and blocks biological activity of all human interferon alpha subtypes
Clonality
Polyclonal
Host
Sheep
Isotype
Serum
Applications for Human IFN-alpha Antibody
Application
Recommended Usage
Neutralization
One neutralization unit is the amount of antiserum required to neutralize one unit of human interferon alpha
(Hu-IFN-alpha 2) to a 50% endpoint. Interferon was titrated with the use of the cytopathic effect inhibition assay as described [Rubinstein, S., Familletti, P.C., and Pestka, S. (1981) "Convenient Assay for Interferons," J. Virol. 37, 755-758; Familletti, P.C., Rubinstein, S., and Pestka, S. (1981) "A Convenient and Rapid Cytopathic Effect Inhibition Assay for Interferon," in Methods in Enzymology, Vol. 78 (S. Pestka, ed.), Academic Press, New York, 387-394]. In this antiviral assay for interferon about 1 unit/ml of interferon is the quantity necessary to produce a cytopathic effect of 50%. The units are determined with respect to the international reference standard for Hu-IFN-alpha provided by the National Institutes of Health [see Pestka, S. (1986) "Interferon Standards and General Abbreviations," in Methods in Enzymology (S. Pestka, ed.), Academic Press, New York 119, 14-23]. This material is prepared specifically for effective neutralization of Hu-IFN-alpha.
(Hu-IFN-alpha 2) to a 50% endpoint. Interferon was titrated with the use of the cytopathic effect inhibition assay as described [Rubinstein, S., Familletti, P.C., and Pestka, S. (1981) "Convenient Assay for Interferons," J. Virol. 37, 755-758; Familletti, P.C., Rubinstein, S., and Pestka, S. (1981) "A Convenient and Rapid Cytopathic Effect Inhibition Assay for Interferon," in Methods in Enzymology, Vol. 78 (S. Pestka, ed.), Academic Press, New York, 387-394]. In this antiviral assay for interferon about 1 unit/ml of interferon is the quantity necessary to produce a cytopathic effect of 50%. The units are determined with respect to the international reference standard for Hu-IFN-alpha provided by the National Institutes of Health [see Pestka, S. (1986) "Interferon Standards and General Abbreviations," in Methods in Enzymology (S. Pestka, ed.), Academic Press, New York 119, 14-23]. This material is prepared specifically for effective neutralization of Hu-IFN-alpha.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
N/A
Formulation
Neat serum diluted in phosphate buffered saline (PBS)
Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
After receipt, the product may be stored at -20°C for short-term use (≤ 6 months). For long-term storage, we recommend storing the product at -70°C or below for retention of full activity. When thawing, the contents of the tube should be apportioned in separate tubes so that freezing and thawing is kept to a minimum. Refreezing should be done on dry ice or in a dry ice/alcohol bath. Further dilution of the product should be in buffers containing protein such as 0.1% bovine serum albumin (BSA).
Background: IFN-alpha
Long Name
Interferon alpha
Alternate Names
IFNalpha, LeIF D
Entrez Gene IDs
3440 (Human)
Gene Symbol
IFNA2
Additional IFN-alpha Products
Product Documents for Human IFN-alpha Antibody
Product Specific Notices for Human IFN-alpha Antibody
For research use only
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