Human IFN-beta Antibody
R&D Systems, part of Bio-Techne | Catalog # 31401-1
Key Product Details
Species Reactivity
Human
Applications
Neutralization
Label
Unconjugated
Antibody Source
Polyclonal Sheep Serum
Product Specifications
Immunogen
Recombinant human IFN‑ beta 1a (expressed in mammalian cells)
Specificity
Detects human IFN‑ beta.
Clonality
Polyclonal
Host
Sheep
Isotype
Serum
Applications for Human IFN-beta Antibody
Application
Recommended Usage
Neutralization
One neutralization unit is the amount of antiserum required to neutralize one unit of
human interferon beta (Hu-IFN-beta) to a 50% endpoint. Interferon was titrated with the use of the cytopathic effect inhibition
assay as described [Rubinstein, S., Familletti, P.C., and Pestka, S. (1981) "Convenient Assay for Interferons," J. Virol. 37,
755-758; Familletti, P.C., Rubinstein, S., and Pestka, S. (1981) "A Convenient and Rapid Cytopathic Effect Inhibition
Assay for Interferon," in Methods in Enzymology, Vol. 78 (S. Pestka, ed.), Academic Press, New York, 387-394]. In this
antiviral assay for interferon about 1 unit/ml of interferon is the quantity necessary to produce a cytopathic effect of 50%.
The units are determined with respect to the international reference standard for Hu-IFN-beta provided by the National
Institutes of Health [see Pestka, S. (1986) "Interferon Standards and General Abbreviations," in Methods in Enzymology
(S. Pestka, ed.), Academic Press, New York 119, 14-23]. This material is prepared specifically for effective neutralization
of Hu-IFN-beta.
Formulation, Preparation, and Storage
Purification
N/A
Formulation
Neat serum.
Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
After receipt, the product may be stored at -20°C for short-term use (≤ 6 months). For
long-term storage, we recommend storing the product at -70°C or below for retention of full activity. When thawing, the
contents of the tube should be apportioned in separate tubes so that freezing and thawing is kept to a minimum.
Refreezing should be done on dry ice or in a dry ice/alcohol bath. Further dilution of the product should be in buffers
containing protein such as 0.1% bovine serum albumin (BSA).
Background: IFN-beta
Long Name
Interferon beta
Alternate Names
IFB, IFF, IFNB, IFNB1, IFNbeta
Gene Symbol
IFNB1
Additional IFN-beta Products
Product Documents for Human IFN-beta Antibody
Product Specific Notices for Human IFN-beta Antibody
For research use only
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