Human IFN-gamma R1/CD119 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB6732
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
Neutralization
Cited:
Blocking, Neutralization
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # GIR208
Product Specifications
Immunogen
Human placenta-derived IFN‑ gamma R1/CD119
Specificity
Detects human IFN‑ gamma R1/CD119 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse IFN-gamma R1, recombinant human (rh) IFN-gamma R2, or rhIL-10 R beta is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human IFN-gamma R1/CD119 Antibody
IFN‑ gamma Inhibition of EMCV-induced Cytopathy and Neutralization by Human IFN‑ gamma R1/CD119 Antibody.
Recombinant Human IFN-gamma (Catalog # 285-IF) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line in a dose-dependent manner (orange line). Inhibition of EMCV activity elicited by Recombinant Human IFN-gamma (2 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IFN-gamma R1/CD119 Mono-clonal Antibody (Catalog # MAB6732). The ND50 is typically 0.03-0.15 µg/mL.Applications for Human IFN-gamma R1/CD119 Antibody
Application
Recommended Usage
Neutralization
Measured by its ability to neutralize IFN-gamma R1/CD119-mediated inhibition of EMCV-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line. The Neutralization Dose (ND50) is typically 0.03-0.15 µg/mL in the presence of 2 ng/mL Recombinant Human IFN-gamma.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IFN-gamma R1/CD119
The high-affinity IFN-gamma receptor complex is made up of two type I membrane proteins, IFN-gamma R1 (IFN-gamma R alpha) and IFN-gamma R2 (IFN-gamma R beta). Both proteins are members of the type II cytokine receptor family and share approximately 52% amino acid sequence identity. IFN-gamma R1 is the ligand-binding subunit that is necessary and sufficient for IFN-gamma binding and receptor internalization. IFN-gamma R2 is required for IFN-gamma signaling but does not bind IFN-gamma by itself. Human IFN-gamma R1 cDNA encodes a 499 amino acid (aa) protein with a 17 aa signal peptide, a 228 aa extracellular domain, a 23 aa transmembrane domain, and a 221 aa intracellular domain. Human and mouse IFN-gamma R1 share 52% aa identity and bind IFN-gamma in a species-specific manner. IFN-gamma R1 is constitutively expressed in most cell types. Soluble IFN-gamma R1 that binds IFN-gamma has been detected in biological fluids.
References
- Bach, E.A. et al. (1997) Annu. Rev. Immunol. 15:563.
Long Name
Interferon gamma Receptor 1
Alternate Names
CD119, IFN-gR1, IFNgammaR1, IFNGR1
Gene Symbol
IFNGR1
Additional IFN-gamma R1/CD119 Products
Product Documents for Human IFN-gamma R1/CD119 Antibody
Product Specific Notices for Human IFN-gamma R1/CD119 Antibody
For research use only
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