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Human IFN-gamma  R2 Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAF773

R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human IFN-gamma R2
Ala22-Gln247
Accession # P38484

Specificity

Detects human IFN‑ gamma R2 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human (rh) IFN-gamma RI and rhIL‑10 R beta is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human IFN-gamma  R2 Biotinylated Antibody

Detection of IFN-? R2 antibody in THP-1 Human Cell Line antibody by Flow Cytometry.

Detection of IFN‑ gamma R2 in THP‑1 Human Cell Line by Flow Cytometry.

THP-1 human acute monocytic leukemia cell line was stained with Goat Anti-Human IFN-gamma R2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF773, filled histogram) or isotype control antibody (Catalog # BAF108, open histogram), followed by Streptavidin-Allophycocyanin (Catalog # F0050).

Applications for Human IFN-gamma  R2 Biotinylated Antibody

Application
Recommended Usage

Flow Cytometry

0.25 µg/106 cells
Sample: THP-1 human acute monocytic leukemia cell line

Western Blot

0.1 µg/mL
Sample: Recombinant Human IFN-gamma  R2

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IFN-gamma R2

Interferon gamma Receptor 2 (IFN-gamma R2) is a type II transmembrane protein that associates with IFN-gamma R1 to form a high affinity receptor complex for IFN-gamma. IFN‑ gamma R2 is required for signal transduction but does not bind IFN-gamma by itself.

Long Name

Interferon gamma Receptor 2

Alternate Names

AF-1, IFN-gR2, IFNgammaR2, IFNGR2, IFNGT1

Entrez Gene IDs

3460 (Human); 15980 (Mouse)

Gene Symbol

IFNGR2

UniProt

Additional IFN-gamma R2 Products

Product Documents for Human IFN-gamma  R2 Biotinylated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human IFN-gamma  R2 Biotinylated Antibody

For research use only

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