Human IFN-gamma R2 Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF773
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, Western Blot
Label
Biotin
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human IFN-gamma R2
Ala22-Gln247
Accession # P38484
Ala22-Gln247
Accession # P38484
Specificity
Detects human IFN‑ gamma R2 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human (rh) IFN-gamma RI and rhIL‑10 R beta is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human IFN-gamma R2 Biotinylated Antibody
Detection of IFN‑ gamma R2 in THP‑1 Human Cell Line by Flow Cytometry.
THP-1 human acute monocytic leukemia cell line was stained with Goat Anti-Human IFN-gamma R2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF773, filled histogram) or isotype control antibody (Catalog # BAF108, open histogram), followed by Streptavidin-Allophycocyanin (Catalog # F0050).Applications for Human IFN-gamma R2 Biotinylated Antibody
Application
Recommended Usage
Flow Cytometry
0.25 µg/106 cells
Sample: THP-1 human acute monocytic leukemia cell line
Sample: THP-1 human acute monocytic leukemia cell line
Western Blot
0.1 µg/mL
Sample: Recombinant Human IFN-gamma R2
Sample: Recombinant Human IFN-gamma R2
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IFN-gamma R2
Interferon gamma Receptor 2 (IFN-gamma R2) is a type II transmembrane protein that associates with IFN-gamma R1 to form a high affinity receptor complex for IFN-gamma. IFN‑ gamma R2 is required for signal transduction but does not bind IFN-gamma by itself.
Long Name
Interferon gamma Receptor 2
Alternate Names
AF-1, IFN-gR2, IFNgammaR2, IFNGR2, IFNGT1
Gene Symbol
IFNGR2
UniProt
Additional IFN-gamma R2 Products
Product Documents for Human IFN-gamma R2 Biotinylated Antibody
Product Specific Notices for Human IFN-gamma R2 Biotinylated Antibody
For research use only
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