Human IgG Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB11012
Recombinant Monoclonal Antibody.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 1268C
Product Specifications
Immunogen
Purified IgG from human serum
Specificity
Detects human IgG in direct ELISAs. As a pan-Human IgG-specific antibody, sandwich immunoassay specificity is determined by the capture antibody.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human IgG Antibody
Human IgG1ELISA Standard Curve.
Recombinant Human IgG1protein was serially diluted 2-fold and captured by Mouse Anti-Human IgG1Monoclonal Antibody (Catalog # MAB9894) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rabbit Anti-Human IgG Monoclonal Antibody (Catalog # MAB11012) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Human IgG2ELISA Standard Curve.
Recombinant Human IgG2protein was serially diluted 2-fold and captured by Mouse Anti-Human IgG2Monoclonal Antibody (Catalog # MAB9794) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rabbit Anti-Human IgG Monoclonal Antibody (Catalog # MAB11012) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Human IgG4ELISA Standard Curve.
Recombinant Human IgG4protein was serially diluted 2-fold and captured by Mouse Anti-Human IgG4Monoclonal Antibody (Catalog # MAB9895) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rabbit Anti-Human IgG Monoclonal Antibody (Catalog # MAB11012) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).Applications for Human IgG Antibody
Application
Recommended Usage
ELISA
This antibody functions as a Human IgG ELISA detection antibody when paired with Mouse Anti-Human IgG1 Monoclonal Antibody (Catalog # MAB9894), Rabbit Anti-Human IgG1 Monoclonal Antibody (Catalog # MAB9930), Mouse Anti-Human IgG2 Monoclonal Antibody (Catalog # MAB9794), Rabbit Anti-Human IgG2 Monoclonal Antibody (Catalog # MAB97941), Mouse Anti-Human IgG4 Monoclonal Antibody (Catalog # MAB9895), Rabbit Anti-Human IgG4 Monoclonal Antibody (Catalog # MAB98951), or Human IgG Monoclonal Antibody (Catalog # MAB11013).
This product is intended for assay development on various assay platforms requiring antibody pairs.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IgG
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
Long Name
Immunoglobulin G
Alternate Names
Immunoglobulin G, ImmunoglobulinG
Additional IgG Products
Product Documents for Human IgG Antibody
Product Specific Notices for Human IgG Antibody
For research use only
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