Human IGSF4C/SynCAM4 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB41641
Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Specifications
Immunogen
Gln25-Tyr323
Accession # Q8NFZ8
Specificity
Clonality
Host
Isotype
Scientific Data Images for Human IGSF4C/SynCAM4 Antibody
IGSF4C/SynCAM4 in Human Kidney.
IGSF4C/SynCAM4 was detected in immersion fixed paraffin-embedded sections of human kidney array using Rat Anti-Human IGSF4C/ SynCAM4 Monoclonal Antibody (Catalog # MAB41641) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human IGSF4C/SynCAM4 Antibody
Immunohistochemistry
Sample: Immersion fixed paraffin-embedded sections of human kidney subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)
Formulation, Preparation, and Storage
Purification
Reconstitution
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IGSF4C/SynCAM4
IGSF4C is an immunoglobulin superfamily member that is also known as Nectin-like protein 4 (Necl-4), synaptic cell adhesion molecule 4 (SynCAM4), or tumor suppressor in lung cancer-like 2 (TSLL-2) (1, 2). The four IGSF4 proteins, designated A, B, C and D, are type I transmembrane glycoproteins expressed mainly in neurons, but also in lung, kidney, bladder, prostate and testis (1‑5). Their extracellular domains (ECD) share 35‑50% amino acid (aa) identity and each contain one V‑type Ig-like and two C2-type Ig-like domains. These domains are responsible for Ca++‑independent homophilic and heterophilic interactions. The 388 aa human IGSF4C contains a 20 aa signal sequence, a 304 aa ECD that shares 98‑99% amino acid identity with mouse, rat, canine and bovine IGSF4C, a 21 aa transmembrane sequence, and a 43 aa cytoplasmic domain. The apparent size of mouse or human IGSF4C may be variably reported as 48‑67 kDa, probably due to differences in glycosylation (2, 5, 8). In the peripheral nervous system, IGSF4C is expressed on Schwann cells, and its internodal interaction with IGSF4A (Necl-1, SynCAM-3) on axons is critical for adhesion and myelination (6‑8). In the brain, all IGSF4 family members are expressed at high levels concurrent with synapse formation (4). In the cerebellum, IGSF4C is expressed on Purkinje cells, with complementary expression of IGSF4 on granule cells (4). Heterophilic interaction with IGSF4D (Necl-3, SynCAM2) has also been identified, but homophilic interaction is unlikely (4, 6). IGSF4C is also proposed as a tumor suppressor that is downregulated in many prostate cancers and gliomas (1, 5).
References
- Fukuhara, H. et al. (2001) Oncogene 20:5401.
- Biederer, T. et al. (2006) Genomics 87:139.
- Takai, Y. et al. (2008) Nat. Rev. Mol. Cell Biol. 9:603.
- Thomas, L. A. et al. (2008) J. Comp. Neurol. 510:47.
- Williams, Y. N. et al. (2005) Oncogene 25:1446.
- Fogel, A. I. et al. (2007) J. Neurosci. 27:12516.
- Spiegel, I. et al. (2007) Nat. Neurosci. 10:861.
- Maurel, P. et al. (2007) J. Cell Biol. 178:861.
Long Name
Alternate Names
Gene Symbol
UniProt
Additional IGSF4C/SynCAM4 Products
Product Documents for Human IGSF4C/SynCAM4 Antibody
Product Specific Notices for Human IGSF4C/SynCAM4 Antibody
For research use only