Human IKK epsilon Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3199
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human IKK epsilon
Met1-Val716
Accession # Q14164
Met1-Val716
Accession # Q14164
Specificity
Detects human IKK epsilon in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human IKK epsilon Antibody
Detection of Human IKK epsilon by Western Blot.
Western blot shows lysates of MDA-MB-468 human breast cancer cell line, Raji human Burkitt's lymphoma cell line, and Ramos human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human IKKe Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3199) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for IKKe at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
IKK epsilon in Ramos Human Cell Line.
IKKe was detected in immersion fixed Ramos human Burkitt's lymphoma cell line using Goat Anti-Human IKKe Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3199) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Human IKK epsilon Antibody
Application
Recommended Usage
Immunocytochemistry
10-25 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells and Ramos human Burkitt's lymphoma cell line
Sample: Immersion fixed human peripheral blood mononuclear cells and Ramos human Burkitt's lymphoma cell line
Western Blot
1 µg/mL
Sample: MDA-MB-468 human breast cancer cell line, Raji human Burkitt's lymphoma cell line, and Ramos human Burkitt's lymphoma cell line
Sample: MDA-MB-468 human breast cancer cell line, Raji human Burkitt's lymphoma cell line, and Ramos human Burkitt's lymphoma cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IKK epsilon
Long Name
IkB Kinase epsilon
Alternate Names
IKBKE, IKK-I
Gene Symbol
IKBKE
UniProt
Additional IKK epsilon Products
Product Documents for Human IKK epsilon Antibody
Product Specific Notices for Human IKK epsilon Antibody
For research use only
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