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Human IL-1 RII Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB263

R&D Systems, part of Bio-Techne
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MAB263
MAB263-SP

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry, Neutralization, Western Blot

Cited:

Cell Selection, Immunocytochemistry, Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 32437

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-1 RII
Phe14-Glu343 (Glu297Gly)
Accession # P27930

Specificity

Detects human IL-1 RII in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL-1 RI, rhIL-18 R, and recombinant mouse IL-18 R is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human IL-1 RII Antibody

IL‑1 RII Inhibition of IL‑1 beta/IL‑1F2‑dependent Cell Proliferation and Neutralization by Human IL‑1 RII Antibody.

IL‑1 RII Inhibition of IL‑1 beta/IL‑1F2‑dependent Cell Proliferation and Neutralization by Human IL‑1 RII Antibody.

Recombinant Human IL-1 RII (263-2R) inhibits Recombinant Human IL-1 beta/ IL-1F2 (201-LB) induced proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human IL-1 beta/IL-1F2 (50 pg/mL) activity elicited by Recombinant Human IL-1 RII (2 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-1 RII Monoclonal Antibody (Catalog # MAB263). The ND50 is typically 2-6 µg/mL.

Detection of IL-1 RII in HDLM-2 (positive) and K562 (negative).

IL-1 RII was detected in immersion fixed HDLM-2 human Hodgkin’s lymphoma cells (positive) and absent in K562 human chronic myelogenous leukemia cells (negative) using Mouse Anti-Human IL-1 RII Monoclonal Antibody (Catalog # MAB263) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surface. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Detection of IL-1 RII in HDLM-2 cells by Flow Cytometry

HDLM-2 cells were stained with Mouse Anti-Human IL-1 RII Monoclonal Antibody (Catalog # MAB263, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Applications for Human IL-1 RII Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HDLM-2 human Hodgkin's lymphoma cells (positive) and absent in K562 human chronic myelogenous leukemia cells (negative)

Western Blot

1 µg/mL
Sample: Recombinant Human IL‑1 RII (Catalog # 263-2R) under non-reducing conditions only

Neutralization

Measured by its ability to neutralize IL‑1 RII-mediated inhibition of proliferation in the D10.G4.1 mouse helper T cell line. The Neutralization Dose (ND50) is typically 2-6 µg/mL in the presence of 2 µg/mL Recombinant Human IL‑1 RII, 50 pg/mL Recombinant Human IL‑1 beta/IL‑1F2.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-1 RII

Two distinct types of receptors that bind the pleiotropic cytokines IL-1 alpha and IL-1 beta have been described. The IL-1 receptor type I is an 80 kDa transmembrane protein that is expressed predominantly by T cells, fibroblasts and endothelial cells. IL-1 receptor type II is a 68 kDa transmembrane protein found on B lymphocytes, neutrophils, monocytes, large granular leukocytes and endothelial cells. Both receptors are members of the immunoglobulin superfamily and show approximately 28% sequence similarity in their extracellular domains. The two receptor types do not heterodimerize in a receptor complex.

An IL-1 receptor accessory protein that can heterodimerize with the type I receptor in the presence of IL-1 alpha or IL-1 beta but not IL-1ra, was identified (1). This type I receptor complex appears to mediate all the known IL-1 biological responses. The receptor type II has a short cytoplasmic domain and does not transduce IL-1 signals. In addition to the membrane-bound form of IL-1 RII, a naturally-occurring soluble form of IL-1 RII has been described. It has been suggested that the type II receptor, either as the membrane-bound or as the soluble form, serves as a decoy for IL-1 and inhibits IL-1 action by blocking the binding of IL-1 to the signaling type I receptor complex.

References

  1. Greenfeder, S. et al. (1995) J. Biol. Chem. 270:13757.

Long Name

Interleukin 1 Receptor II

Alternate Names

CD121b, IL-1 R beta, IL-1RII, IL1R2, IL1RII

Entrez Gene IDs

7850 (Human); 16178 (Mouse)

Gene Symbol

IL1R2

UniProt

Additional IL-1 RII Products

Product Documents for Human IL-1 RII Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human IL-1 RII Antibody

For research use only

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