Human IL-11 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB218R
Recombinant Monoclonal Antibody.
Key Product Details
Species Reactivity
Human
Applications
Neutralization, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG2A Clone # 22626R
Product Specifications
Immunogen
E. coli-derived recombinant human IL‑11
Pro22-Leu199
Accession # P20809
Pro22-Leu199
Accession # P20809
Specificity
Detects human IL-11 in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human IL-11 Antibody
Cell Proliferation Induced by IL-11 and Neutralization by Human IL-11 Antibody.
Recombinant Human IL-11 (218-IL) stimulates proliferation in the T11 mouse plasmacytoma cell line in a dose-dependent manner (orange line), as measured by Resazurin (AR002). Proliferation elicited by Recombinant Human IL-11 (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-11 Monoclonal Antibody (Catalog # MAB218R). The ND50 is typically 5-15 µg/mLApplications for Human IL-11 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Human IL‑11 (Catalog # 218-IL)
Sample: Recombinant Human IL‑11 (Catalog # 218-IL)
Neutralization
Measured by its ability to neutralize IL‑11-induced proliferation in the T11 mouse plasmacytoma cell line. Nordan, R. P. et al. (1987) J. Immunol. 139:813. The Neutralization Dose (ND50) is typically5-15 µg/mL in the presence of 1 ng/mL Recombinant Human IL‑11.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-11
Long Name
Interleukin 11
Alternate Names
AGIF, IL11, Oprelvekin
Gene Symbol
IL11
UniProt
Additional IL-11 Products
Product Documents for Human IL-11 Antibody
Product Specific Notices for Human IL-11 Antibody
For research use only
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