Human peripheral blood mononuclear cells (PBMCs) either (A) treated with 5 µg/mL PHA for 48 hours (B) untreated, were stained with Mouse Anti-Human IL-12 R beta1 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB839F) and Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (Catalog # FAB100A). Quadrant markers were set based on control antibody staining (Catalog # IC002F). View our protocol for Staining Membrane-associated Proteins.

Applications for Human IL-12 R beta1 Fluorescein-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: Human peripheral blood mononuclear cells (PBMCs) treated with PHA

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: IL-12 R beta 1

Interleukin12 (IL-12) is a key mediator of cellular-immunity and induces the differentiation of Th1 cells from precursor T helper cells. The biological activities of IL-12 are mediated through the high-affinity receptor complex composed of two subunits designated IL-12 R beta1 and IL-12 R beta2. Individually, IL-12 R beta1 and IL-12 R beta2 bind IL‑12 with low affinity. Co-expression of both subunits confers high-affinity binding and is required for IL-12 activity. Both IL-12 receptor subunits are type I transmembrane proteins that share similarities with the gp130/G-CSF R subgroup in the cytokine receptor superfamily. IL-12 R beta1 cDNA encodes a 662 amino acid (aa) protein with a putative 23 aa signal peptide that is cleaved to generate the mature protein with a 522 aa extracellular domain, a 25 aa transmembrane domain and a 92 aa cytoplasmic region. Expression of IL-12 R beta1 is detected in activated T cells, NK cells and B cells. The expression of IL‑12 R beta2 is more restricted and appears to be limited to Th2 cells.

References

Gately, M.K. et al. (1998) Annu. Rev. Immunol. 16:495.

Long Name

Interleukin 12 Receptor beta 1

Alternate Names

CD212, IL-12Rb1, IL12R beta 1, IL12RB1

Entrez Gene IDs

3594 (Human); 16161 (Mouse)

Gene Symbol

IL12RB1

UniProt

P42701

Additional IL-12 R beta 1 Products

Product Documents for Human IL-12 R beta1 Fluorescein-conjugated Antibody

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COA

SDS

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Product Specific Notices for Human IL-12 R beta1 Fluorescein-conjugated Antibody

For research use only

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Flow Cytometry

Human IL-12 R beta1 Fluorescein-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB839F

Key Product Details

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Species Reactivity

Validated:

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Validated:

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Label

Antibody Source

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Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human IL-12 R beta1 Fluorescein-conjugated Antibody

Detection of IL-12 R beta1 in Human PBMCs by Flow Cytometry.