Human IL-17F Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB13353

Recombinant Monoclonal Antibody.
R&D Systems, part of Bio-Techne
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MAB13353-100
MAB13353-SP
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Key Product Details

Species Reactivity

Human

Applications

ELISA

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG2B Clone # 775602R

View all IL-17F Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human IL-17F
Gly21-Thr153
Accession # Q96PD4

Specificity

Detects human IL-17F in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human IL-17F Antibody

Human IL-17F Antibody in ELISA Standard Curve.

Human IL‑17F ELISA Standard Curve.

Recombinant Human IL-17F protein was serially diluted 2-fold and captured by Mouse Anti-Human IL-17F Monoclonal Antibody (Catalog # MAB13354) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human IL-17F Monoclonal Antibody (Catalog # MAB13353) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Applications for Human IL-17F Antibody

Application
Recommended Usage

ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human IL‑17F Monoclonal Antibody (Catalog # MAB13354).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human IL-17F DuoSet ELISA Kit (Catalog # DY1335B-05) for convenient development of a sandwich ELISA.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-17F

The Interleukin 17 (IL‑17) family proteins, comprising six members (IL‑17A through IL‑17F), are secreted, structurally related proteins that share a conserved cystine‑knot fold near the C-terminus, but have considerable sequence divergence at the N‑terminus. With the exception of IL‑17B, which exists as a non‑covalently linked dimer, all IL-17 family members are disulfide-linked dimers. IL‑17 family proteins are pro-inflammatory cytokines that induce local cytokine production and are involved in the regulation of immune functions (1, 2).Human IL‑17F cDNA encodes a 163 aa protein with a putative 30 aa signal peptide. Among IL‑17 family members, IL‑17F is most closely related to IL-17A (approximately 44% aa sequence homology), but shares only limited sequence homology (16-30%) with IL‑17B, C, D and E. Human and mouse IL‑17F share 55% sequence identity. IL-17F is expressed in activated CD4+ T cells and activated monocytes. Five receptors (IL-17 RA, B, C, D and E) have been identified (5). Although the ligands for IL-17 RD and E are not known yet, it is reported that IL-17 RA binds IL-17A, and IL-17 RB binds
IL-17B and IL-17E. IL-17 RC binds IL-17A and IL‑17F with similarly high affinity and functions as a receptor for both IL-17A and IL-17F (5, 6). The biological activities mediated by IL‑17F are similar to those of IL‑17. IL‑17F stimulates production of IL‑6, IL‑8, G‑CSF, and regulates cartilage matrix turnover by increasing matrix release and inhibiting new matrix synthesis (4). IL‑17F also inhibits angiogenesis and induces production of IL‑2, TGF-beta, and monocyte chemoattractant protein‑1 in endothelial cells (3).

References

  1. Aggarwal, S. and A.L. Gurney (2002) J. Leukoc. Biol. 71:1.
  2. Moseley, T.A. et al. (2003) Cytokine & Growth Factor Rev. 14:155.
  3. Starnes, T. et al. (2001) J. Immunol. 167:4137.
  4. Shen, F. & S. L. Gaffen (2008) Cytokine 41:92.
  5. Kuestner, R.E. et al. (2007) J. Immunol. 179:5462.

Long Name

Interleukin 17F

Alternate Names

IL17F

Entrez Gene IDs

112744 (Human); 257630 (Mouse); 301291 (Rat); 708220 (Rhesus Macaque)

Gene Symbol

IL17F

UniProt

Q96PD4

Additional IL-17F Products

Product Documents for Human IL-17F Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human IL-17F Antibody

For research use only

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