Human IL-17RB Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAF1207

R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
BAF1207
Print Quote
Bulk Order
Conjugate
Catalog #
Unconjugated
AF1207
View Product
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG

View all IL-17RB Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human IL‑17 RB
Arg18-Gly289
Accession # NP_061195

Specificity

Detects human IL‑17 RB in Western blots. In Western blots, approximately 35% cross-reactivity with recombinant mouse (rm) IL-17 RB is observed, 5% cross-reactivity with rmIL-17 R is observed, and less than 1% cross-reactivity with recombinant human IL-17 R is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human IL-17RB Biotinylated Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human kidney

Western Blot

0.1 µg/mL
Sample: Recombinant Human IL‑17 RB Fc Chimera (Catalog # 1207-BR)

Reviewed Applications

Read 1 review rated 5 using BAF1207 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-17RB

The interleukin 17 (IL-17) family of cytokines, comprising six members (IL-17, IL-17B through IL-17F), are structurally related proteins with a conserved cysteine-knot structure. These proinflammatory cytokines can induce local cytokine productions and are involved in the regulation of the immune response. The cognate receptors activated by some of these cytokines have been identified (1). Interleukin-17 B Receptor (IL-17 RB), also known as IL-17Rh1, IL-17ER and EVI27, represents the second receptor of the IL-17 family of cytokines to be recognized (2-4). Human IL-17 RB cDNA encodes a 502 amino acid (aa) residue type I membrane protein with a putative 17 aa signal peptide, a 275 aa extracellular domain, a 21 aa transmembrane domain and a 189 aa cytoplasmic tail. By alternative splicing, a secreted variant of IL-17 RB has also been identified (4). Human and mouse IIL-17 RB share 76% aa sequence identity. The human IL-17 RB protein sequence is only 19.2% identical to the human IL-17 R sequence, but the two receptors share many conserved cysteine residues within their extracellular domains as well as additional conserved elements within their cytoplasmic domains. Three additional type I transmembrane receptors with homology to IL-17 R have been reported, increasing the number of the IL-17 R family members to five (5, 6). By Northern blot analysis, human IL-17 RB is highly expressed in kidneys and liver but is expressed at lower levels in testes, brain, small intestine and other endocrine tissues (2-4). The expression of IL-17 RB is significantly up-regulated under inflammatory conditions. IL-17 RB binds strongly to IL-17E and weakly to IL-17B. It does not bind IL-17, IL-17C, and IL-17F. Activation of IL-17 RB by its ligands results in the activation of nuclear factor kappa-B (2-4).

References

  1. Aggarwal, S. and A.L. Gurney (2002) J. Leukoc. Biol. 71:1.
  2. Shi Y, et al. (2000) J. Biol. Chem. 275:19167.
  3. Lee, J, et al. (2001) J. Biol. Chem. 276:1660.
  4. Tian E, et al. (2000) Oncogene 19:2098.
  5. Haudenschild, D. et al. (2002) J. Biol. Chem. 277:4309.
  6. Hurst, S.D. et al. (2002) J. Immunol. 169:443.

Long Name

Interleukin 17B Receptor

Alternate Names

Evi27, IL-17 RB, IL-17 RH1, IL-17ER, IL17RB

Entrez Gene IDs

55540 (Human); 50905 (Mouse)

Gene Symbol

IL17RB

UniProt

NP_061195

Additional IL-17RB Products

Product Documents for Human IL-17RB Biotinylated Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human IL-17RB Biotinylated Antibody

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.