Human IL-18 R alpha/IL-1 R5 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF840
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Rat
Applications
Validated:
CyTOF-ready, Flow Cytometry, Immunohistochemistry, Neutralization, Western Blot
Cited:
Bioassay, Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human IL‑18 R alpha/IL-1 R5
Glu20-Arg329
Accession # Q13478
Glu20-Arg329
Accession # Q13478
Specificity
Detects human IL‑18 R alpha/IL-1 R5 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant mouse IL‑18 R alpha is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human IL-18 R alpha/IL-1 R5 Antibody
IL-18 R alpha/IL-1 R5 in Human Skin.
IL-18 Ra/IL-1 R5 was detected in immersion fixed frozen sections of human skin using Goat Anti-Human IL-18 Ra/IL-1 R5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF840) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS009) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane of keratinocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
IFN-gamma Secretion Induced by IL-18/IL-1F4 and Neutralization by Human IL-18 R alpha/IL-1 R5 Antibody.
Recombinant Human IL-18/IL-1F4 stimulates IFN-gamma secretion in the KG-1 human acute myelogenous leukemia cell line, in a dose-dependent manner (orange line), as measured by the Human IFN-gamma Quantikine ELISA Kit (Catalog # DIF50C). IFN-gamma secretion elicited by IL-18/IL-1F4 (40 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-18 Ra/IL-1 R5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF840). The ND50 is typically 0.1-0.4 µg/mL.Applications for Human IL-18 R alpha/IL-1 R5 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: Human CD3+ T cells treated with PHA and Recombinant Human IL‑2 (Catalog # 202-IL)
Sample: Human CD3+ T cells treated with PHA and Recombinant Human IL‑2 (Catalog # 202-IL)
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed frozen sections of human skin
Sample: Immersion fixed frozen sections of human skin
Western Blot
0.1 µg/mL
Sample: Recombinant Human IL-18 R alpha/IL-1 R5 Fc Chimera (Catalog # 816-LR)
Sample: Recombinant Human IL-18 R alpha/IL-1 R5 Fc Chimera (Catalog # 816-LR)
Neutralization
Measured by its ability to neutralize IL-18/IL-1F4-induced IFN-gamma secretion in the KG-1 human acute myelogenous leukemia cell line. The Neutralization Dose (ND50) is typically 0.1-0.4 µg/mL in the presence of 40 ng/mL Recombinant Human IL-18/IL-1F4.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-18 R alpha/IL-1 R5
Interleukin 18 (IL-18) is a member of the IL-1 family of cytokines and shares numerous immuno-regulatory functions with IL-12. The functional IL-18 receptor complex is composed of two subunits designated IL-18 R alpha (also termed IL-1 R5 and IL-1 Rrp) and IL-18 R beta (also termed IL-1 R7 and AcPL). Both IL-18 R alpha and IL-18 R beta belong to the IL-1 receptor superfamily. Although IL-18 R by itself binds IL-18 with low-affinity and IL-18 R beta does not bind IL-18 in vitro, co-expression of IL-18 R alpha and IL‑18 R beta is required for high-affinity binding and IL-18 responsiveness. Human IL-18 R cDNA encodes a 541 amino acid (aa) precursor type I membrane protein with a hydrophobic signal, an extracellular domain comprised of three immunoglobulin-like domains, a transmembrane domain and a cytoplasmic region of approximately 200 aa residues. Human and mouse IL-18 R share 65% amino acid sequence homology. IL-18 R is widely expressed in numerous tissues including spleen, thymus, leukocyte, liver, lung, heart, small and large intestine, prostate, and placenta.
References
- Parnet, P. et al. (1996) J. Biol. Chem. 271:3967.
- Torigoe, K. et al. (1997) J. Biol. Chem. 272:25737.
- Born, T.L. et al. (1998) J. Biol. Chem. 273:29445.
Long Name
Interleukin 18 Receptor alpha
Alternate Names
CD218a, IL-1 R5, IL-1 Rrp, IL-18 R1, IL-18Ra, IL18R alpha, IL18R1
Gene Symbol
IL18R1
UniProt
Additional IL-18 R alpha/IL-1 R5 Products
Product Documents for Human IL-18 R alpha/IL-1 R5 Antibody
Product Specific Notices for Human IL-18 R alpha/IL-1 R5 Antibody
For research use only
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