Human IL-19 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF1035
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
CyTOF-ready, Intracellular Staining by Flow Cytometry, Neutralization, Western Blot
Cited:
ELISA Development, Immunohistochemistry-Frozen
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human IL-19
Leu25-Ala177 (Phe175Ser)
Accession # Q9UHD0
Leu25-Ala177 (Phe175Ser)
Accession # Q9UHD0
Specificity
Detects human IL‑19 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human IL-19 Antibody
Cell Proliferation Induced by IL‑19 and Neutralization by Human IL‑19 Antibody.
Recombinant Human IL-19 (Catalog # 1035-IL) stimulates proliferation in the BaF3 mouse pro-B cell line co-transfected with human IL-20 Ra and IL-20 R beta in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-19 (3 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-19 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1035). The ND50 is typically 1-3 µg/mL.Applications for Human IL-19 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
Sample: Human peripheral blood mononuclear cells fixed with paraformaldehyde and permeabilized with saponin
Sample: Human peripheral blood mononuclear cells fixed with paraformaldehyde and permeabilized with saponin
Western Blot
0.1 µg/mL
Sample: Recombinant Human IL-19 (Catalog # 1035-IL)
Sample: Recombinant Human IL-19 (Catalog # 1035-IL)
Neutralization
Measured by its ability to neutralize IL-19-induced proliferation in the BaF3 mouse pro-B cell line co-transfected with human IL-20 R alpha and IL-20 R beta. The Neutralization Dose (ND50) is typically 1-3 µg/mL in the presence of 3 ng/mL Recombinant Human IL-19.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-19
Human Interleukin 19 (IL-19) is a member of the IL-10 family of related cytokines. Its gene contains two alternate translation initiation sites, generating precursors of 215 amino acids (aa) and 177 aa, respectively. Both isoforms are processed to 17 kDa, 153 aa mature molecules. IL-19 contains seven helices and is secreted as a 35 kDa monomer. There are two potential N-linked glycosylation sites, and it is likely that the molecule is glycosylated. Mature human IL-19 shares 69% aa sequence identity with the mature mouse homologue. Although mouse IL-19 is active on human cells, human IL-19 is not active on mouse cells. IL-19 expression is limited to activated keratinocytes and monocytes. IL-19 binds a receptor complex consisting of the IL-20 receptor alpha (IL-20 R alpha, also known as IL-20 R1) and the IL-20 receptor beta (IL-20 R beta or IL-20 R2). This receptor complex is also shared by IL-20 and IL-24. Functionally, IL-19 induces IL-6 and TNF-alpha production by monocytes, and drives T-helper cell differentiation towards a Th2 response (1‑5).
References
- Gallagher, G. et al. (2000) Genes Immun. 1:442.
- Gallagher, G. et al. (2004) Int. Immunopharmacol. 4:615.
- Laio, Y-C. et al. (2002) J. Immunol. 169:4288.
- Romer, J. et al. (2003) J. Invest. Dermatol. 121:1306.
- Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929.
Long Name
Interleukin 19
Alternate Names
IL19
Gene Symbol
IL19
UniProt
Additional IL-19 Products
Product Documents for Human IL-19 Antibody
Product Specific Notices for Human IL-19 Antibody
For research use only
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