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Human IL-1 alpha/IL-1F1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB2003

R&D Systems, part of Bio-Techne
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MAB2003-100
MAB2003-SP

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 1042708

Product Specifications

Immunogen

E. coli-derived human IL-1 alpha/IL-1F1 protein
Ser113-Ala271
Accession # Q53QF9

Specificity

Detects human IL-1 alpha/Il-1F1 in direct ELISAs

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human IL-1 alpha/IL-1F1 Antibody

IL-1 alpha/IL-1F1 in RT-4 Human Cell Line.

IL-1 alpha/IL-1F1 was detected in immersion fixed RT-4 human urinary bladder transitional cell papilloma cell line (positive staining) and THP-1 human acute monocytic leukemia cell line (negative staining) using Mouse Anti-Human IL-1 alpha/IL-1F1 Monoclonal Antibody (Catalog # MAB2003) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human IL-1 alpha/IL-1F1 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed RT‑4 human urinary bladder transitional cell papilloma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-1 alpha/IL-1F1

Interleukin 1 (IL-1) is a name that designates two proteins, IL-1 alpha and IL-1 beta, which are the products of distinct genes, but which show approximately 25% amino acid sequence identity and which recognize the same cell surface receptors. Although IL-1 production is generally considered to be a consequence of inflammation, recent evidence suggests that IL-1 is also temporarily upregulated during bone formation and the menstrual cycle and can be induced in response to nervous system stimulation. In response to classic stimuli produced by inflammatory agents, infections or microbial endotoxins, a dramatic increase in the production of IL-1 by macrophages and various other cells is seen. Cells in particular known to produce IL-1 include osteoblasts, monocytes, macrophages, keratinocytes, Kupffer cells, hepatocytes, thymic and salivary gland epithelium, Schwann cells, fibroblasts and glia (oligodendroglia, astrocytes and microglia).

IL-1 alpha and IL-1 beta are both synthesized as 31 kDa precursors that are subsequently cleaved into proteins with molecular weights of approximately 17,000 Da. Neither precursor contains a typical hydrophobic signal peptide sequence and most of the precursor form of IL-1 alpha remains in the cytosol of cells, although there is evidence for a membrane-bound form of the precursor form of IL-1 alpha. The IL-1 alpha precursor reportedly shows full biological activity in the EL-4 assay. Among various species, the amino acid sequence of mature IL-1 alpha is conserved 60% to 70% and human IL-1 has been found to be biologically active on murine cell lines. Both forms of IL-1 bind to the same receptors, designated type I and type II. Evidence suggests that only the type I receptor is capable of signal transduction and that the type II receptor may function as a decoy, binding IL-1 and thus preventing binding of IL-1 to the type I receptor.

Long Name

Interleukin 1 alpha

Alternate Names

BAF, IL-1A, IL-1F1, IL1, IL1 alpha, IL1A, IL1F1, LAF, LEM

Entrez Gene IDs

3552 (Human); 16175 (Mouse); 24493 (Rat); 397094 (Porcine); 101926546 (Cynomolgus Monkey)

Gene Symbol

IL1A

UniProt

Additional IL-1 alpha/IL-1F1 Products

Product Documents for Human IL-1 alpha/IL-1F1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human IL-1 alpha/IL-1F1 Antibody

For research use only

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