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Human IL-2 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB2022

R&D Systems, part of Bio-Techne
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MAB2022-100
MAB2022-SP

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 5325

Product Specifications

Immunogen

E. coli-derived recombinant human IL‑2
Ala21-Thr153
Accession # NP_000577

Specificity

Detects human IL-2 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human IL-2 Antibody

IL-2 antibody in Human Stomach by Immunohistochemistry (IHC-P).

IL-2 in Human Stomach.

IL-2 was detected in immersion fixed paraffin-embedded sections of human stomach using Mouse Anti-Human IL-2 Monoclonal Antibody (Catalog # MAB2022) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to intestinal glands. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human IL-2 Antibody

Application
Recommended Usage

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human stomach tissue

Reviewed Applications

Read 1 review rated 5 using MAB2022 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-2

Interleukin 2 (IL-2) is a cytokine that stimulates the growth and differentiation of B cells, T cells, NK cells, and monocyte/macrophages. It functions through the heterotrimeric IL-2 receptor comprising alpha, beta, and gamma chains.

Long Name

Interleukin 2

Alternate Names

Aldesleukin, IL2, Proleukin, TCGF

Entrez Gene IDs

3558 (Human); 16183 (Mouse); 116562 (Rat); 396868 (Porcine); 280822 (Bovine); 403989 (Canine); 100034204 (Equine); 751114 (Feline); 100302458 (Rabbit)

Gene Symbol

IL2

UniProt

Additional IL-2 Products

Product Documents for Human IL-2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human IL-2 Antibody

For research use only

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