Human IL-2 R beta Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB2241
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Simple Western, Western Blot
Cited:
Flow Cytometry, Neutralization
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 27311
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-2 R beta
Ala27-Asp239
Accession # NP_000869
Ala27-Asp239
Accession # NP_000869
Specificity
Detects human IL-2 R beta in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human (rh) IL-2 R alpha, recombinant mouse IL-2 R beta, rhIL-2 R gamma, rhIL-15 R alpha, or rhIL-21 R is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human IL-2 R beta Antibody
Detection of Human IL-2 R beta by Simple WesternTM.
Simple Western lane view shows lysates of HDLM-2 human Hodgkin’s lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for IL-2 R beta at approximately 98 kDa (as indicated) using 25 µg/mL of Mouse Anti-Human IL-2 R beta Monoclonal Antibody (Catalog # MAB2241) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human IL-2 R beta Antibody
Application
Recommended Usage
Simple Western
25 µg/mL
Sample: HDLM-2 human Hodgkin's lymphoma cell line
Sample: HDLM-2 human Hodgkin's lymphoma cell line
Western Blot
1 µg/mL
Sample: Recombinant Human IL-2 R beta (Catalog # 224-2B)
Sample: Recombinant Human IL-2 R beta (Catalog # 224-2B)
Reviewed Applications
Read 1 review rated 4 using MAB2241 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-2 R beta
Functional IL-2 receptors can exist in two affinity states on cell surfaces, the high affinity complex consisting of heterotrimers of the alpha, beta, and gamma chains, and the intermediate affinity complex comprising heterodimers of the beta and gamma chains. Individual beta chains and alpha chains exhibit low affinity IL-2 binding and the gamma chain alone does not bind IL-2. In addition to their involvement in IL-2 mediated signal transduction, both the beta chain and gamma chain are required for IL-15 mediated signaling.
IL-2 R beta is a member of the cytokine receptor superfamily. Human IL-2 R beta cDNA encodes a 551 amino acid (aa) precursor Type I transmembrane protein with a 26 aa signal peptide, a 214 aa extracellular region, a 25 aa transmembrane region and a 286 aa cytoplasmic domain. A soluble IL-2 R beta (IL-2 sR beta) has been identified in the culture supernatants of a human lymphoid cell line, YT, that displays IL-2 R beta.
Long Name
Interleukin 2 Receptor beta
Alternate Names
CD122, IL-15 R beta, IL-2Rb, IL2R beta, IL2RB
Gene Symbol
IL2RB
UniProt
Additional IL-2 R beta Products
Product Documents for Human IL-2 R beta Antibody
Product Specific Notices for Human IL-2 R beta Antibody
For research use only
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