Human IL-22 R alpha1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF2770
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Neutralization
Cited:
Blocking, Immunohistochemistry-Frozen, Neutralization, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human IL-22 R alpha1
Pro18-Thr228
Accession # Q8N6P7
Pro18-Thr228
Accession # Q8N6P7
Specificity
Detects human IL‑22 R alpha1 in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant human IL-20 R beta is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human IL-22 R alpha1 Antibody
IL‑10 secretion Induced by IL‑22 and Neutralization by Human IL‑22 R alpha1 Antibody.
Recombinant Human IL-22 (Catalog # 782-IL) stimulates IL-10 secretion in the COLO 205 human colorectal adenocarcin-oma cell line in a dose-dependent manner (orange line), as measured by the Human IL-10 DuoSet ELISA Development Kit (Catalog # DY217B). IL-10 secretion elicited by Recombinant Human IL-22 (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-22 Ra1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2770). The ND50 is typically 0.2-1 µg/mL.
Detection of Human IL-22R alpha 1 by Flow Cytometry
Stimulation of AGS cells with IL-22 leads to the inhibition of H. pylori-induced CCL20 expression.A, AGS cells were stained with an isotype control antibody (dashed line) or an anti-IL-22R1 antibody (solid line) conjugated with allophycocyanin and analyzed by flow cytometry. B, AGS cells were infected with H. pylori (HP) in the presence of various concentration of IL-22 for 24 h and the CCL20 concentration in the culture supernatants was determined by ELISA. C-D, AGS cells were infected with H. pylori in the presence or absence of IL-22 and the CCL20 mRNA in the cells (C) and CCL20 protein in the culture supernatants (D) were determined by real-time PCR and ELISA, respectively. E. AGS cells were pretreated with a neutralizing antibody against IL-22R1 (2 µg/ml) for 1 h followed by the H. pylori infection in the absence or presence of IL-22 for 6 h. The CCL20 concentration in the culture supernatants was determined by ELISA. Data represent the mean ± SEM from three independent experiments. **, p<0.005; *, p<0.02 for H. pylori + IL-22 versus H. pylori only. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0097350), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human IL-22R alpha 1 by Western Blot
IL-22 and IL-22 receptor expression levels increase in the airways of asthmatic subjects. (A-D) Bronchial biopsies were obtained from healthy controls, mild asthmatics and severe asthmatics and stained (A, negative control) for IL-22 expression (in brown) by immunohistochemistry. Scale bar 50 μm. (E) The number of IL-22 positive cells was determined per mm2 of biopsy tissue. n=5 per group, *p<0.05 vs. healthy control. (F) Primary bronchial epithelial cells were obtained from healthy controls, mild asthmatics and severe asthmatics and assessed for IL-22 receptor expression by Western blot. Cells were allowed to grow to confluence, serum starved overnight and stimulated with IL-22, TGF-beta 1 (10 ng/mL each) or both cytokines for 5 days before Western blot analysis. (G) Expression levels of the IL-22 receptor in unstimulated cells were quantified relative to GAPDH expression. n=5 per group, *p<0.05 vs. healthy control. Image collected and cropped by CiteAb from the following publication (https://respiratory-research.biomedcentral.com/articles/10.1186/1465-9921-14-118), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Human IL-22 R alpha1 Antibody
Application
Recommended Usage
Neutralization
Measured by its ability to neutralize IL‑22-induced IL‑10 secretion in the COLO 205 human colorectal adenocarcinoma cell line. Marehalli, L. et al. (2004) Intl. Immunopharmacol. 4:679. The Neutralization Dose (ND50) is typically 0.2-1 µg/mL in the presence of 1 ng/mL Recombinant Human IL‑22.
Reviewed Applications
Read 1 review rated 4 using AF2770 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-22 R alpha 1
IL-22 receptor, also known as IL-22 R alpha1 and CRF2-9, is an approximately 65 kDa transmembrane glycoprotein in the type II cytokine receptor family (CRF). IL-22 R alpha1 contains a 211 amino acid (aa) extracellular domain (ECD) with two fibronectin type III repeats, and a 323 aa cytoplasmic domain. IL-22 R alpha1 associates with either IL-10 R beta or IL-20 R beta to form receptor complexes with distinct ligand selectivities. IL-10 R beta is a shared subunit of the IL-10, -22, -26, -28, and -29 receptors, while IL-20 R beta is a shared subunit of the IL-19, -20, -22R and -24 receptors (1). IL-22 R alpha1/IL-10 R beta is an IL-22 responsive receptor (2, 3), and IL-22 R alpha1/IL-20 R beta is an IL-20 or IL-24 responsive receptor (4, 5). IL-22 R alpha1 contains cytoplasmic motifs for interactions with signal transduction molecules, but formation of ternary complexes with IL-10 R beta or IL-20 R beta and the respective ligands is required for signal transduction (2, 6). IL-22BP functions as a competitive antagonist by binding
IL‑22 and preventing its association with IL-22 R alpha1 (7, 9). Even though it is a receptor for interleukins, IL-22 R alpha1 is not expressed on hematopoietic cells (6, 10, 11). Instead, IL-22 R alpha1 expression is restricted to epithelial and stromal cells (6, 10‑13). IL-22 R alpha1 signaling promotes innate immune responses and wound healing at sites of infection and inflammation. This includes upregulation of antimicrobial, acute phase, proinflammatory, and extracellular matrix proteins as well as proteases (3, 11, 13, 14). IL-22 R alpha1 signaling also promotes downregulation of proteins involved in keratinocyte differentiation (3, 14). Within the ECD, human IL-22 R alpha1 shares 78%, 76%, and 83% aa sequence identity with mouse, rat, and canine IL-22 R, respectively. It shares 22% - 25% aa sequence identity with the ECDs of other class II receptors IL-10 R, IL-20 R, and IL-28 R.
References
- Langer, J.A. et al. (2004) Cytokine Growth Factor Rev. 15:33.
- Xie, M.-H. et al. (2000) J. Biol. Chem. 275:31335.
- Boniface, K. et al. (2005) J. Immunol. 174:3695.
- Dumoutier, L. et al. (2001) J. Immunol. 167:3545.
- Wang, M. et al. (2002) J. Biol. Chem. 277:7341.
- Kotenko, S.V. et al. (2001) J. Biol. Chem. 276:2725.
- Li, J. et al. (2004) Int. Immunopharmacol. 4:693.
- Logsdon, N.J. et al. (2002) J. Interferon Cytokine Res. 22:1099.
- Kotenko, S.V. et al. (2001) J. Immunol. 166:7096.
- Nagalakshmi, M.L. et al. (2004) Int. Immunopharmacol. 4:577.
- Nagalakshmi, M.L. et al. (2004) Int. Immunopharmacol. 4:679.
- Aggarwal, S. et al. (2001) J. Interferon Cytokine Res. 21:1047.
- Wolk, K. et al. (2004) Immunity 21:241.
- Wolk, K. et al. (2006) Eur. J. Immunol. 36:1309.
Long Name
Interleukin 22 Receptor
Alternate Names
CRF2-9, IL-22Ra1, IL-TIF-R1, IL22R alpha 1, IL22RA1
Gene Symbol
IL22RA1
UniProt
Additional IL-22 R alpha 1 Products
Product Documents for Human IL-22 R alpha1 Antibody
Product Specific Notices for Human IL-22 R alpha1 Antibody
For research use only
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