Daudi human Burkitt's lymphoma cell line was stained with Mouse Anti-Human IL-28 Ra/IFN-lambda R1 PE-conjugated Monoclonal Antibody (Catalog # FAB5260P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Detection of IL‑28 R alpha/IFN-lambda R1 in PBMCs by Flow Cytometry.

Peripheral blood mononuclear cells (PBMCs) either untreated (A) or treated (B) with 50 ng/mL PMA and 200 ng/mL calcium ionomycin overnight were stained with Mouse Anti-Human IL-28 Ra/IFN-lambda R1 PE-conjugated Monoclonal Antibody (Catalog # FAB5260P) and Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (Catalog # FAB100A). Quadrant markers were set based on control antibody staining (Catalog # IC002P). View our protocol for Staining Membrane-associated Proteins.

Applications for Human IL-28 R alpha/IFN-lambda R1 PE-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: Daudi Human Burkitt's Lymphoma Cell Line and Peripheral blood mononuclear cells (PBMCs) treated with PMA and calcium ionomycin

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: IL-28 R alpha/IFN-lambda R1

IL-28 R alpha (IL-28 Receptor alpha subunit), also known as Interferon-lambda R1 (IFN-lambda R1) is a widely-expressed type I transmembrane glycoprotein that is Cytokine Receptor Family 2 member 12 (CRF2-12). It pairs with the IL-10 receptor beta subunit (IL-10 R beta, CRF2-4) to form the IL-28 R. Its ligands, IL-28A (IFN-lambda 2), IL-28B (IFN-lambda 3), and IL-29 (IFN-lambda 1), are mainly produced by antigen presenting cells in response to viruses. The 520 amino acid (aa) mouse IL-28 R alpha contains a 208 aa extracellular domain (ECD) with a fibronectin type III motif. The human IL-28 R alpha ECD shares ~67% aa identity with rat and human IL‑28 R alpha. Isoforms of 491, 437, 322, 283, 244, 211 and 184 aa have alternate N- or C-termini, or lack transmembrane or internal sequences.

Long Name

Interleukin 28 Receptor alpha

Alternate Names

CRF2-12, IFN-lambda R1, IFNLR1, IL-28Ra, IL28R alpha, IL28RA, LICR2

Entrez Gene IDs

163702 (Human); 242700 (Mouse); 362628 (Rat)

Gene Symbol

IFNLR1

UniProt

Q8IU57 (Human)

Additional IL-28 R alpha/IFN-lambda R1 Products

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Product Datasheets

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COA

SDS

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Product Specific Notices for Human IL-28 R alpha/IFN-lambda R1 PE-conjugated Antibody

For research use only

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Flow Cytometry

Human IL-28 R alpha/IFN-lambda R1 PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB5260P

Key Product Details

Validated by

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

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Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human IL-28 R alpha/IFN-lambda R1 PE-conjugated Antibody

Detection of IL‑28 R alpha/IFN-lambda R1 in Daudi Human Cell Line by Flow Cytometry.